ArticleProceedings of the National Academy of Sciences of the United States of America2020
Sonoselective transfection of cerebral vasculature without blood-brain barrier disruption.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 55 citations in OpenAlex.
- Focused Ultrasound is Poised to Take Non-Viral Gene Therapies to the Blood-Brain Barrier - and Beyond.Cell biomaterials · 2026Article
- Sonoselective Transfection of Glioma Endothelium.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Focused ultrasound-mediated opening of the blood brain barrier for non-invasive drug delivery to the murine cerebellum.Drug delivery and translational research · 2026Article
- Alteration of Essential Cell Function by Ultrasound-Targeted Microbubble Cavitation.Current cardiology reports · 2025Review
- Focused Ultrasound: Noninvasive Image-Guided Therapy.Investigative radiology · 2025Review
- Characterization of focused ultrasound blood-brain barrier disruption effect on inflammation as a function of treatment parameters.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2025Article
- Miniaturized therapeutic systems for ultrasound-modulated drug delivery to the central and peripheral nervous system.Advanced drug delivery reviews · 2024Review
- Bibliometric and visualized analysis of ultrasound combined with microbubble therapy technology from 2009 to 2023.Frontiers in pharmacology · 2024Review
- Sonoselective delivery using ultrasound and microbubbles combined with intravenous rAAV9 CLDN5-GFP does not increase endothelial gene expression.Gene therapy · 2023Article
- The Evolution and Recent Trends in Acoustic Targeting of Encapsulated Drugs to Solid Tumors: Strategies beyond Sonoporation.Pharmaceutics · 2023Review
- Engineered nanomaterials that exploit blood-brain barrier dysfunction for delivery to the brain.Advanced drug delivery reviews · 2023Review
- The blood-brain barrier: structure, regulation, and drug delivery.Signal transduction and targeted therapy · 2023Review
- Single-cell mapping of focused ultrasound-transfected brain.Gene therapy · 2023Article
- Theranostics in the vasculature: bioeffects of ultrasound and microbubbles to induce vascular shutdown.Theranostics · 2023Review
- Applications of focused ultrasound-mediated blood-brain barrier opening.Advanced drug delivery reviews · 2022Review
- An Affordable and Easy-to-Use Focused Ultrasound Device for Noninvasive and High Precision Drug Delivery to the Mouse Brain.IEEE transactions on bio-medical engineering · 2022Article
- The roles of thermal and mechanical stress in focused ultrasound-mediated immunomodulation and immunotherapy for central nervous system tumors.Journal of neuro-oncology · 2022Review
- Ultrasound-mediated gene delivery of factor VIII plasmids for hemophilia A gene therapy in mice.Molecular therapy. Nucleic acids · 2022Article
- Computational model of brain endothelial cell signaling pathways predicts therapeutic targets for cerebral pathologies.Journal of molecular and cellular cardiology · 2022Article
- A sporadic Alzheimer's blood-brain barrier model for developing ultrasound-mediated delivery of Aducanumab and anti-Tau antibodies.Theranostics · 2022Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Treatment of many pathologies of the brain could be improved markedly by the development of noninvasive therapeutic approaches that elicit robust, endothelial cell-selective gene expression in specific brain regions that are targeted under MR image guidance. While focused ultrasound (FUS) in conjunction with gas-filled microbubbles (MBs) has emerged as a noninvasive modality for MR image-guided gene delivery to the brain, it has been used exclusively to transiently disrupt the blood-brain barrier (BBB), which may induce a sterile inflammation response. Here, we introduce an MR image-guided FUS method that elicits endothelial-selective transfection of the cerebral vasculature (i.e., "sonoselective" transfection), without opening the BBB. We first determined that activating circulating, cationic plasmid-bearing MBs with pulsed low-pressure (0.1 MPa) 1.1-MHz FUS facilitates sonoselective gene delivery to the endothelium without MRI-detectable disruption of the BBB. The degree of endothelial selectivity varied inversely with the FUS pressure, with higher pressures (i.e., 0.3-MPa and 0.4-MPa FUS) consistently inducing BBB opening and extravascular transfection. Bulk RNA sequencing analyses revealed that the sonoselective low-pressure regimen does not up-regulate inflammatory or immune responses. Single-cell RNA sequencing indicated that the transcriptome of sonoselectively transfected brain endothelium was unaffected by the treatment. The approach developed here permits targeted gene delivery to blood vessels and could be used to promote angiogenesis, release endothelial cell-secreted factors to stimulate nerve regrowth, or recruit neural stem cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.