ArticleDevelopment (Cambridge, England)2020
Physical interactions between Gsx2 and Ascl1 balance progenitor expansion versus neurogenesis in the mouse lateral ganglionic eminence.
Article in Development (Cambridge, England), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- An inducible system to study the regulatory functions of GSX2 in human lateral ganglionic eminence-like progenitors.Developmental biology · 2026Article
- Gsx2 regulates oligodendrocyte precursor formation in the zebrafish spinal cord.Developmental biology · 2026Article
- Neocortical neurogenesis: a proneural gene perspective.The FEBS journal · 2025Review
- Gsx2 Regulates Oligodendrocyte Precursor Formation in the Zebrafish Spinal Cord.bioRxiv : the preprint server for biology · 2025Article
- 3D cultured human medium spiny neurons functionally integrate and rescue motor deficits in Huntington's disease mice.The Journal of clinical investigation · 2025Article
- Modelling a pathological GSX2 variant that selectively alters DNA binding reveals hypomorphic mouse brain defects.Disease models & mechanisms · 2025Article
- Modeling early phenotypes of Parkinson's disease by age-induced midbrain-striatum assembloids.Communications biology · 2024Article
- Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface.Nucleic acids research · 2024Article
- Prediction of cooperative homeodomain DNA binding sites from high-throughput-SELEX data.Nucleic acids research · 2023Article
- Gsx2, but not Gsx1, is necessary for early forebrain patterning and long-term survival in zebrafish.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- FGF8-FGFR1 signaling regulates human GnRH neuron differentiation in a time- and dose-dependent manner.Disease models & mechanisms · 2022Article
- Single cell enhancer activity distinguishes GABAergic and cholinergic lineages in embryonic mouse basal ganglia.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Olig2 defines a subset of neural stem cells that produce specific olfactory bulb interneuron subtypes in the subventricular zone of adult mice.Development (Cambridge, England) · 2022Article
- Function of Proneural Genes Ascl1 and Asense in Neurogenesis: How Similar Are They?Frontiers in cell and developmental biology · 2022Review
- Genetic Regulation of Vertebrate Forebrain Development by Homeobox Genes.Frontiers in neuroscience · 2022Review
- From Progenitors to Progeny: Shaping Striatal Circuit Development and Function.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Review
- Temporally Distinct Roles for the Zinc Finger Transcription Factor Sp8 in the Generation and Migration of Dorsal Lateral Ganglionic Eminence (dLGE)-Derived Neuronal Subtypes in the Mouse.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- Environmental and Molecular Modulation of Motor Individuality in Larval Zebrafish.Frontiers in behavioral neuroscience · 2021Article
- Conserved Gsx2/Ind homeodomain monomer versus homodimer DNA binding defines regulatory outcomes in flies and mice.Genes & development · 2021Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
The Gsx2 homeodomain transcription factor promotes neural progenitor identity in the lateral ganglionic eminence (LGE), despite upregulating the neurogenic factor Ascl1. How this balance in maturation is maintained is unclear. Here, we show that Gsx2 and Ascl1 are co-expressed in subapical progenitors that have unique transcriptional signatures in LGE ventricular zone (VZ) cells. Moreover, whereas Ascl1 misexpression promotes neurogenesis in dorsal telencephalic progenitors, the co-expression of Gsx2 with Ascl1 inhibits neurogenesis. Using luciferase assays, we found that Gsx2 reduces the ability of Ascl1 to activate gene expression in a dose-dependent and DNA binding-independent manner. Furthermore, Gsx2 physically interacts with the basic helix-loop-helix (bHLH) domain of Ascl1, and DNA-binding assays demonstrated that this interaction interferes with the ability of Ascl1 to bind DNA. Finally, we modified a proximity ligation assay for tissue sections and found that Ascl1-Gsx2 interactions are enriched within LGE VZ progenitors, whereas Ascl1-Tcf3 (E-protein) interactions predominate in the subventricular zone. Thus, Gsx2 contributes to the balance between progenitor maintenance and neurogenesis by physically interacting with Ascl1, interfering with its DNA binding and limiting neurogenesis within LGE progenitors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.