ArticleAnimals : an open access journal from MDPI2020
Effects of Acute Heat Stress on a Newly Established Chicken Hepatocyte-Nonparenchymal Cell Co-Culture Model.
Article in Animals : an open access journal from MDPI, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 25 citations in OpenAlex.
- Investigating the effects of thymol and cinnamaldehyde in T-2 toxin-treated hepatic cell cultures of chicken origin.Poultry science · 2026Article
- The Pro-Inflammatory Action of Different Pathogen-Associated Molecular Patterns in Porcine Hepatic and Intestinal Cell Cultures.Animals : an open access journal from MDPI · 2026Article
- Role of T-2 toxin in the modulation of oxidative homeostasis and immune function in three-dimensional hepatic cell cultures of chicken origin.Frontiers in veterinary science · 2026Article
- Hepatocyte production across embryonic stages in chicken: an in vitro approach.BMC veterinary research · 2025Article
- Protective effects of baicalin against deoxynivalenol-induced oxidative and inflammatory damage in chicken-derived hepatic 3D cell cultures.Scientific reports · 2025Article
- Miniature chicken ileal explant culture to investigate the inflammatory response induced by pathogen-associated molecular patterns.Frontiers in veterinary science · 2025Article
- Investigation of the effects of T-2 toxin in chicken-derived three-dimensional hepatic cell cultures.Scientific reports · 2024Article
- Pap12-6: A host defense peptide with potent immunomodulatory activity in a chicken hepatic cell culture.PloS one · 2024Article
- Cecropin A: investigation of a host defense peptide with multifaceted immunomodulatory activity in a chicken hepatic cell culture.Frontiers in veterinary science · 2024Article
- Modulation of the immune response by the host defense peptide IDR-1002 in chicken hepatic cell culture.Scientific reports · 2023Article
- Luteolin: A Phytochemical to MitigateAnimals : an open access journal from MDPI · 2023Article
- Feline Uroepithelial Cell Culture as a Novel Model of Idiopathic Cystitis: Investigations on the Effects of Norepinephrine on Inflammatory Response, Oxidative Stress, and Barrier Function.Veterinary sciences · 2023Article
- Mechanisms underlying the Effects of Heat Stress on Intestinal Integrity, Inflammation, and Microbiota in Chickens.The journal of poultry science · 2023Review
- Molecular effects of intermittent stress on primary feline uroepithelial cell culture as anFrontiers in veterinary science · 2023Article
- Mechanisms Underlying the Protective Effect of Maternal Zinc (ZnSOAntioxidants (Basel, Switzerland) · 2022Article
- Effect of Heat Stress on Egg Production, Steroid Hormone Synthesis, and Related Gene Expression in Chicken Preovulatory Follicular Granulosa Cells.Animals : an open access journal from MDPI · 2022Article
- Negative effects of heat stress on ovarian tissue in female rabbit.Frontiers in veterinary science · 2022Article
- Immunomodulatory effects of chicken cathelicidin-2 on a primary hepatic cell co-culture model.PloS one · 2022Article
- Zinc alleviates the heat stress of primary cultured hepatocytes of broiler embryos via enhancing the antioxidant ability and attenuating the heat shock responses.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
- Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Heat stress is one of the most important issues in broiler flocks impairing animal health and productivity. On a cellular level, excess heat exposure can trigger heat shock response acting for the restoration of cell homeostasis by several mechanisms, such as affecting heat shock protein synthesis, redox homeostasis and pro-inflammatory cytokine production. The major aim of this study was to establish a novel avian hepatocyte-nonparenchymal cell co-culture as a model for investigating the cellular effects of heat stress and its interaction with inflammation in chicken liver. Cell fractions were isolated by differential centrifugation from a freshly perfused chicken liver, and hepatocyte mono-cultures as well as hepatocyte-nonparenchymal cell co-cultures (with cell ratio 6:1, hepatocytes to nonparenchymal cells, mimicking a milder hepatic inflammation) were prepared. Isolated and cultured cells were characterized by flow cytometry and immunocytochemistry applying hepatocyte- and macrophage-specific antibodies. Confluent cell cultures were exposed to 43 °C temperature for 1 or 2 h, while controls were cultured at 38.5 °C. The metabolic activity, LDH enzyme activity, reactive oxygen species (H
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.