Evidence map›Paper›PMID 32121340›Full record

ArticleNanomaterials (Basel, Switzerland)2020

Mineral-Doped Poly(L-lactide) Acid Scaffolds Enriched with Exosomes Improve Osteogenic Commitment of Human Adipose-Derived Mesenchymal Stem Cells.

Maria Giovanna Gandolfi, Chiara Gardin, Fausto Zamparini, Letizia Ferroni, Micaela Degli Esposti, Greta Parchi, Batur Ercan, Lucia Manzoli, Fabio Fava, Paola Fabbri and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanomaterials (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
57citing papers in PubMed, 1 pooled it
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

57 citing papers in PubMed, 1 synthesis or guideline pooled it, 80 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Advanced small extracellular vesicles delivery systems forExtracellular vesicles and circulating nucleic acids · 2026
    Review
  4. Review
  5. Review
  6. Review
  7. Review
  8. Review
  9. Exosome-Based Therapeutics in Dermatology.Biomaterials research · 2025
    Review
  10. Review
  11. Article
  12. Review
  13. Role of Adipose-Derived Mesenchymal Stem Cells in Bone Regeneration.International journal of molecular sciences · 2024
    Review
  14. Review
  15. Article
  16. Review
  17. Review
  18. Delivery of Growth Factors to Enhance Bone Repair.Bioengineering (Basel, Switzerland) · 2023
    Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Maria Giovanna GandolfiLaboratory of Biomaterials and Oral Pathology, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40125 Bologna, Italy.
Chiara GardinMedical Sciences Department, University of Ferrara, 44100 Ferrara, Italy.
Fausto ZampariniLaboratory of Biomaterials and Oral Pathology, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40125 Bologna, Italy.
Letizia FerroniMedical Sciences Department, University of Ferrara, 44100 Ferrara, Italy.
Micaela Degli EspostiDepartment of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, 40136 Bologna, Italy.
Greta ParchiLaboratory of Biomaterials and Oral Pathology, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40125 Bologna, Italy.
Batur ErcanDepartment of Metallurgical and Materials Engineering, 06800 Ankara, Turkey.
Lucia ManzoliCellular Signaling Laboratory, Institute of Human Anatomy, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40126 Bologna, Italy.
Fabio FavaDepartment of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, 40136 Bologna, Italy.
Paola FabbriDepartment of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, 40136 Bologna, Italy.
Carlo PratiEndodontic Clinical Section, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40125 Bologna, Italy.
Barbara ZavanMedical Sciences Department, University of Ferrara, 44100 Ferrara, Italy.
University of Bologna · ITUniversity of Ferrara · ITMiddle East Technical University · TR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exosomes derived from mesenchymal stem cells are extracellular vesicles released to facilitate cell communication and function. Recently, polylactic acid (PLA), calcium silicates (CaSi), and dicalcium phosphate dihydrate (DCPD) have been used to produce bioresorbable functional mineral-doped porous scaffolds-through thermally induced phase separation technique, as materials for bone regeneration. The aim of this study was to investigate the effect of mineral-doped PLA-based porous scaffolds enriched with exosome vesicles (EVs) on osteogenic commitment of human adipose mesenchymal stem cells (hAD-MSCs). Two different mineral-doped scaffolds were produced: PLA-10CaSi-10DCPD and PLA-5CaSi-5DCPD. Scaffolds surface micromorphology was investigated by ESEM-EDX before and after 28 days immersion in simulated body fluid (HBSS). Exosomes were deposited on the surface of the scaffolds and the effect of exosome-enriched scaffolds on osteogenic commitment of hAD-MSCs cultured in proximity of the scaffolds has been evaluated by real time PCR. In addition, the biocompatibility was evaluated by direct-contact seeding hAD-MSCs on scaffolds surface-using MTT viability test. In both formulations, ESEM showed pores similar in shape (circular and elliptic) and size (from 10-30 µm diameter). The porosity of the scaffolds decreased after 28 days immersion in simulated body fluid. Mineral-doped scaffolds showed a dynamic surface and created a suitable bone-forming microenvironment. The presence of the mineral fillers increased the osteogenic commitment of hAD-MSCs. Exosomes were easily entrapped on the surface of the scaffolds and their presence improved gene expression of major markers of osteogenesis such as collagen type I, osteopontin, osteonectin, osteocalcin. The experimental scaffolds enriched with exosomes, in particular PLA-10CaSi-10DCPD, increased the osteogenic commitment of MSCs. In conclusion, the enrichment of bioresorbable functional scaffolds with exosomes is confirmed as a potential strategy to improve bone regeneration procedures.

Indexed as

bioresorbable scaffoldcalcium silicatesexosome enriched scaffoldsexosomesgreen bioplasticshuman adipose mesenchymal stem cells (hAD-MSCs), polylactic acid scaffoldnanodeliverynatural nanovesiclespersonalized regenerative medicine.regenerative bone healing

Identifiers

PMID32121340
PMCPMC7153699
OpenAlexW3010363329

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.