ArticleBiosensors2020
Design of Multiplex Lateral Flow Tests: A Case Study for Simultaneous Detection of Three Antibiotics.
Article in Biosensors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- A Rational Optimization Approach for the Development of a Multiplexed Lateral Flow Immunoassay: Detection of Nonepithelial Ovarian Cancer Markers in Human Serum.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Breaking barriers through driving innovation: sensitivity enhancement strategies for lateral flow assays.Mikrochimica acta · 2025Review
- Double Lateral Flow Test System for Simultaneous Immunodetection of Enantiomeric Forms of Antibiotics: An Ofloxacin Case Study.Biosensors · 2025Article
- Development of a Multiplex Lateral Flow Immunoassay for the Detection of Antibiotics in Milk Utilizing Lyophilized Gold Nanoparticle Conjugates.Biosensors · 2025Article
- Multiplex immunochromatographic assay using a universal labeling reactant for determining antibiotic residues in milk.Veterinary world · 2024Article
- Double lateral flow immunosensing of undeclared pork and chicken components of meat products.Journal of food science and technology · 2024Article
- Lateral flow assay: a promising rapid point-of-care testing tool for infections and non-communicable diseases.Asian biomedicine : research, reviews and news · 2023Review
- An ultrasensitive NIR-IIa' fluorescence-based multiplex immunochromatographic strip test platform for antibiotic residues detection in milk samples.Journal of advanced research · 2023Article
- Biosensors Based on Inorganic Composite Fluorescent Hydrogels.Nanomaterials (Basel, Switzerland) · 2023Review
- Handling Detection Limits of Multiplex Lateral Flow Immunoassay by Choosing the Order of Binding Zones.Micromachines · 2023Article
- Review
- Evaluation of Host Serum Protein Biomarkers of Tuberculosis in sub-Saharan Africa.Frontiers in immunology · 2021Article
- Europium Fluorescent Nanoparticles-Based Multiplex Lateral Flow Immunoassay for Simultaneous Detection of Three Antibiotic Families Residue.Frontiers in chemistry · 2021Article
- A Comparative Study of Approaches to Improve the Sensitivity of Lateral Flow Immunoassay of the Antibiotic Lincomycin.Biosensors · 2020Article
- Switching from Multiplex to Multimodal Colorimetric Lateral Flow Immunosensor.Sensors (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The presented study is focused on the impact of binding zone location on immunochromatographic test strips on the analytical parameters of multiplex lateral flow assays. Due to non-equilibrium conditions for such assays the duration of immune reactions influences significantly the analytical parameters, and the integration of several analytes into one multiplex strip may cause an essential decrease of sensitivity. To choose the best location for binding zones, we have tested reactants for immunochromatographic assays of lincomycin, chloramphenicol, and tetracycline. The influence of the distance to the binding zones on the intensity of coloration and limit of detection (LOD) was rather different. Basing on the data obtained, the best order of binding zones was chosen. In comparison with non-optimal location the LODs were 5-10 fold improved. The final assay provides LODs 0.4, 0.4 and 1.0 ng/mL for lincomycin, chloramphenicol, and tetracycline, respectively. The proposed approach can be applied for multiplexed assays of other analytes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.