ArticleMolecular human reproduction2020
Zinc exocytosis is sensitive to myosin light chain kinase inhibition in mouse and human eggs.
Article in Molecular human reproduction, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- What we could not have known: ovarian cycles, follicle development, and the signature of life.Molecular human reproduction · 2026Review
- The oocyte zinc transportereLife · 2025Article
- Article
- Impact of aging on gene expression in human oocytes: a comparative analysis of young and older patients.Reproductive biology and endocrinology : RB&E · 2025Article
- Article
- The TRPV3 channel is a mediator of zinc influx and homeostasis in murine oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Phenogenetics of cortical granule dynamics during zebrafish oocyte-to-embryo transition.Frontiers in cell and developmental biology · 2025Review
- Molecular Mechanism of Oocyte Activation in Mammals: Past, Present, and Future Directions.Biomolecules · 2024Review
- A tale of two metals: Biofortification of rice grains with iron and zinc.Frontiers in plant science · 2022Review
- Role of zinc in female reproduction.Biology of reproduction · 2021Review
- Knockin' on Egg's Door: Maternal Control of Egg Activation That Influences Cortical Granule Exocytosis in Animal Species.Frontiers in cell and developmental biology · 2021Review
- General Aspects of Metal Ions as Signaling Agents in Health and Disease.Biomolecules · 2020Review
- The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish.Scientific reports · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Zinc dynamics are essential for oocyte meiotic maturation, egg activation, and preimplantation embryo development. During fertilisation and egg activation, the egg releases billions of zinc atoms (Zn2+) in an exocytotic event termed the 'zinc spark'. We hypothesised that this zinc transport and exocytosis is dependent upon the intracellular trafficking of cortical granules (CG) which requires myosin-actin-dependent motors. Treatment of mature mouse and human eggs with ML-7, a myosin light chain kinase inhibitor (MLCK), resulted in an 80% reduction in zinc spark intensity compared to untreated controls when activated with ionomycin. Moreover, CG migration towards the plasma membrane was significantly decreased in ML-7-treated eggs compared with controls when activated parthenogenetically with ionomycin. In sperm-induced fertilisation via intracytoplasmic sperm injection (ICSI), ML-7-treated mouse eggs exhibited decreased labile zinc intensity and cortical CG staining. Collectively, these data demonstrate that ML-7 treatment impairs zinc release from both murine and human eggs after activation, demonstrating that zinc exocytosis requires myosin light chain kinase activity. Further, these results provide additional support that zinc is likely stored and released from CGs. These data underscore the importance of intracellular zinc trafficking as a crucial component of egg maturation necessary for egg activation and early embryo development.
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