ArticlePloS one2020
Efficient immortalization of human dental pulp stem cells with expression of cell cycle regulators with the intact chromosomal condition.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Advances in Regenerative Dentistry: From Biological Mechanisms to Clinical Translation.Life (Basel, Switzerland) · 2026Review
- Engineered Human Dental Pulp Stem Cells with Promising Potential for Regenerative Medicine.Biotech (Basel (Switzerland)) · 2025Review
- Effects of Caffeine on Dental Mesenchymal Stem Cells: Implications for Regenerative Applications.Journal of functional biomaterials · 2025Article
- The Role of Dental-derived Stem Cell-based Therapy and Their Derived Extracellular Vesicles in Post-COVID-19 Syndrome-induced Tissue Damage.Stem cell reviews and reports · 2024Review
- Rejuvenation of human mesenchymal stem cells using a nonintegrative and conditionally removable Sendai virus vector.Scientific reports · 2024Article
- Article
- Research progress on optimization ofFrontiers in bioengineering and biotechnology · 2024Review
- Immortalized cell lines derived from dental/odontogenic tissue.Cell and tissue research · 2023Review
- Immortalized Canine Adipose-Derived Mesenchymal Stem Cells as a Novel Candidate Cell Source for Mesenchymal Stem Cell Therapy.International journal of molecular sciences · 2023Article
- Betaine promotes osteogenic differentiation in immortalized human dental pulp-derived cells.BDJ open · 2022Article
- Combined transgene immortalized urothelial cells capable of reprogramming and hepatic differentiation.Biochemistry and biophysics reports · 2022Article
- 2-hydroxyethyl methacrylate-derived reactive oxygen species stimulate ATP release via TRPA1 in human dental pulp cells.Scientific reports · 2022Article
- Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas.Scientific reports · 2021Article
- Establishment of human airway epithelial cells with doxycycline-inducible cell growth and fluorescence reporters.Cytotechnology · 2021Article
- Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration.International journal of molecular sciences · 2021Review
- Innovative Molecular and Cellular Therapeutics in Cleft Palate Tissue Engineering.Tissue engineering. Part B, Reviews · 2021Review
- Dental Pulp Stem Cells Derived From Adult Human Third Molar Tooth: A Brief Review.Frontiers in cell and developmental biology · 2021Review
- Is There Such a Thing as a Genuine Cancer Stem Cell Marker? Perspectives from the Gut, the Brain and the Dental Pulp.Biology · 2020Review
- Immunomodulation and Regeneration Properties of Dental Pulp Stem Cells: A Potential Therapy to Treat Coronavirus Disease 2019.Cell transplantationReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clinical studies have recently demonstrated that autologous transplantation of mobilized dental pulp stem cells is a safe and efficacious potential therapy for pulp regeneration. However, some limitations need to be addressed, such as the high cost of the safety and quality control tests for isolated individual dental pulp cell products before transplantation. Therefore, more efficient in vitro culturing of human dental pulp stem cells might be useful for providing low cost and high reliability testing for pulp regeneration therapy. In this study, we established a novel immortalized dental pulp stem cell line by co-expressing a mutant cyclin-dependent kinase 4 (CDK4R24C), Cyclin D1, and telomerase reverse transcriptase (TERT). The established cell line maintained its original diploid chromosomes and stemness characteristics and exhibited an enhanced proliferation rate. In addition, we showed the immortalized human dental pulp stem cells still keeps their osteogenic and adipogenic differentiation abilities under appropriate culture conditions even though the cell proliferation was accelerated. Taken together, our established cell lines could serve as a useful in vitro tool for pulp regeneration therapy, and can contribute to reproducibility and ease of cell handling, thereby saving time and costs associated with safety and quality control tests.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.