ArticlePLoS neglected tropical diseases2020
Plasmodium vivax transcriptional profiling of low input cryopreserved isolates through the intraerythrocytic development cycle.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Stage-aware transcriptomics reveals selective haplotype persistence in short-termbioRxiv : the preprint server for biology · 2026Article
- Low-volumeMicrobial genomics · 2025Article
- De novo assembly of plasmodium interspersed repeat (pir) genes from Plasmodium vivax RNAseq data suggests geographic conservation of sub-family transcription.BMC genomics · 2025Article
- Changing Clinical Epidemiology of Plasmodium vivax Malaria as Transmission Decreases: Population-Based Prospective Panel Survey in the Brazilian Amazon.The Journal of infectious diseases · 2024Article
- Comparative transcriptomics reveal differential gene expression among Plasmodium vivax geographical isolates and implications on erythrocyte invasion mechanisms.PLoS neglected tropical diseases · 2024Article
- Development of a Plasmodium vivax biobank for functional ex vivo assays.Malaria journal · 2023Article
- Culture and transfection: Two major bottlenecks in understandingFrontiers in microbiology · 2023Review
- Band 3-mediatedFrontiers in cellular and infection microbiology · 2022Article
- The Truman Show for protozoan parasites: A review of in vitro cultivation platforms.PLoS neglected tropical diseases · 2021Review
- Plasmodium vivax infection compromises reticulocyte stability.Nature communications · 2021Article
- A suitable RNA preparation methodology for whole transcriptome shotgun sequencing harvested from Plasmodium vivax-infected patients.Scientific reports · 2021Article
- On the survival of 48 h Plasmodium vivax Aotus monkey-derived ex vivo cultures: the role of leucocytes filtration and chemically defined lipid concentrate media supplementation.Malaria journal · 2020Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Approximately one-third of the global population is at risk of Plasmodium vivax infection, and an estimated 7.51 million cases were reported in 2017. Although, P. vivax research is currently limited by the lack of a robust continuous in vitro culture system for this parasite, recent work optimizing short-term ex vivo culture of P. vivax from cryopreserved isolates has facilitated quantitative assays on synchronous parasites. Pairing this improved culture system with low-input Smart-seq2 RNAseq library preparation, we sought to determine whether transcriptional profiling of P. vivax would provide insight into the differential survival of parasites in different culture media. To this end we probed the transcriptional signature of three different ex vivo P. vivax samples in four different culture media using only 1000 cells for each time point taken during the course of the intraerythrocytic development cycle (IDC). Using this strategy, we achieved similar quality transcriptional data to previously reported P. vivax transcriptomes. We found little effect with varying culture media on parasite transcriptional signatures, identified many novel gametocyte-specific genes from transcriptomes of FACS-isolated gametocytes, and determined invasion ligand expression in schizonts in biological isolates and across the IDC. In total, these data demonstrate the feasibility and utility of P. vivax RNAseq-based transcriptomic studies using minimal biomass input to maximize experimental capacity.
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