ReviewFrontiers in cell and developmental biology2020
Emerging Methods for Enhancing Pluripotent Stem Cell Expansion.
Review in Frontiers in cell and developmental biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- A clinically defined and xeno-free hydrogel system for regenerative medicine.Materials futures · 2026Article
- The Role of Adenogenesis Factors in the Pathogenesis of Endometriosis.International journal of molecular sciences · 2025Review
- Article
- Hallmarks of totipotent and pluripotent stem cell states.Cell stem cell · 2024Review
- Automated human induced pluripotent stem cell culture and sample preparation for 3D live-cell microscopy.Nature protocols · 2024Review
- Spatial-Temporal Heterogeneity in Large Three-Dimensional Nanofibrillar Cellulose Hydrogel for Human Pluripotent Stem Cell Culture.Gels (Basel, Switzerland) · 2023Article
- Advances in hPSC expansion towards therapeutic entities: A review.Cell proliferation · 2022Review
- Advances in Manufacturing Cardiomyocytes from Human Pluripotent Stem Cells.Annual review of chemical and biomolecular engineering · 2022Review
- Biodegradable Microparticles for Regenerative Medicine: A State of the Art and Trends to Clinical Application.Polymers · 2022Review
- Impact of Musashi-1 and Musashi-2 Double Knockdown on Notch Signaling and the Pathogenesis of Endometriosis.International journal of molecular sciences · 2022Article
- In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture.Communications biology · 2022Article
- The Role of BMP Signaling in Female Reproductive System Development and Function.International journal of molecular sciences · 2021Review
- The effects of surface topography modification on hydrogel properties.APL bioengineering · 2021Review
- Preheating of Gelatin Improves its Printability with Transglutaminase in Direct Ink Writing 3D Printing.International journal of bioprinting · 2020Article
- Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells.Cell transplantationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pluripotent stem cells (PSCs) have great potential to revolutionize the fields of tissue engineering and regenerative medicine as well as stem cell therapeutics. However, the end goal of using PSCs for therapeutic use remains distant due to limitations in current PSC production. Conventional methods for PSC expansion have limited potential to be scaled up to produce the number of cells required for the end-goal of therapeutic use due to xenogenic components, high cost or low efficiency. In this mini review, we explore novel methods and emerging technologies of improving PSC expansion: the use of the two-dimensional mechanobiological strategies of topography and stiffness and the use of three-dimensional (3D) expansion methods including encapsulation, microcarrier-based culture, and suspension culture. Additionally, we discuss the limitations of conventional PSC expansion methods as well as the challenges in implementing non-conventional methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.