ArticleFrontiers in microbiology2020
Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
- Dual-BONCAT reveals distinct subpopulations of anabolically active cells.Applied and environmental microbiology · 2026Article
- Optimizing bio-orthogonal non-canonical amino acid tagging (BONCAT) for low-disruption labeling of Arabidopsis proteins in vivo.Plant physiology · 2026Article
- Anti-discoloration of Tuna Fillet Through the Lactic Acid Bacteria Treatment: Interpretation by Metabolomic Analysis.Probiotics and antimicrobial proteins · 2026Article
- Positive relationship between substrate-induced respiration rate and translationally active bacterial counts in soil.mSystems · 2026Article
- Alternative start codon selection shapes mitochondrial function and rare human diseases.Molecular cell · 2025Article
- Article
- The stringent response does not influence ribosome pausing in Bacillus subtilis.Nucleic acids research · 2025Article
- Using BONCAT to dissect the proteome ofmSphere · 2025Article
- The activity of soil microbial taxa in the rhizosphere predicts the success of root colonization.mSystems · 2025Article
- Engineered Proteins and Materials Utilizing Residue-Specific Noncanonical Amino Acid Incorporation.Chemical reviews · 2024Review
- Bioorthogonal non-canonical amino acid tagging to track transplanted human induced pluripotent stem cell-specific proteome.Stem cell research & therapy · 2024Article
- Detection, isolation and characterization of phage-host complexes using BONCAT and click chemistry.Frontiers in microbiology · 2024Article
- High amino acid osmotrophic incorporation by marine eukaryotic phytoplankton revealed by click chemistry.ISME communications · 2024Article
- Microbial Diversity and Activity of Biofilms from Geothermal Springs in Croatia.Microbial ecology · 2023Article
- Photo-Methionine, Azidohomoalanine and Homopropargylglycine Are Incorporated into Newly Synthesized Proteins at Different Rates and Differentially Affect the Growth and Protein Expression Levels of Auxotrophic and PrototrophicInternational journal of molecular sciences · 2023Article
- Article
- An Integrative Biology Approach to Quantify the Biodistribution of AzidohomoalanineCellular and molecular bioengineering · 2023Article
- Acute stress reduces population-level metabolic and proteomic variation.BMC bioinformatics · 2023Article
- Using click chemistry to study microbial ecology and evolution.ISME communications · 2023Review
- BONCAT-FACS-Seq reveals the active fraction of a biocrust community undergoing a wet-up event.Frontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms. A basic premise of BONCAT is that the non-canonical amino acids (NCAA) used to track translational activity do not significantly alter cellular physiology. If the NCAA would induce changes in the metabolic state of cells, interpretation of BONCAT studies could be challenging. To address this knowledge-gap, we have used a global metabolomics analyses to assess the intracellular effects of NCAA incorporation. Two NCAA were tested:
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.