ArticleFrontiers in microbiology2020
Fifteen-Year Application of Manure and Chemical Fertilizers Differently Impacts Soil ARGs and Microbial Community Structure.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.
- An Overview of Antibiotic Resistance and Abiotic Stresses Affecting Antimicrobial Resistance in Agricultural Soils.International journal of environmental research and public health · 2022Pooled it
- Soil management practices shape the abundance, diversity, and spread of antimicrobial resistance.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Impacts of drought and manure fertilization on soil and radish resistomes.Scientific reports · 2026Article
- Exploring the Relationship Between Farmland Management and Manure-Derived Antibiotic Resistance Genes and Their Prevention and Control Strategies.Antibiotics (Basel, Switzerland) · 2025Review
- A One-Health Perspective of Antimicrobial Resistance (AMR): Human, Animals and Environmental Health.Life (Basel, Switzerland) · 2025Review
- Effects of Antibiotic Residues on Fecal Microbiota Composition and Antimicrobial Resistance Gene Profiles in Cattle from Northwestern China.Microorganisms · 2025Article
- Characterization of the resistome and antibiotic-resistant bacteria in top soil improvers and irrigation waters devoted to food production: a case study from Italy.Environmental science and pollution research international · 2025Article
- Environmental fate of antibiotic resistance genes in livestock farming.Archives of microbiology · 2025Review
- An eco-evolutionary perspective on antimicrobial resistance in the context of One Health.iScience · 2025Review
- Characterization of antibiotic resistance genes in soils from agroecosystems of the Brazilian Amazon.Frontiers in microbiology · 2025Article
- Fertilization impacts microbiomes along the grassland trophic chain.ISME communications · 2025Article
- Integrons in the Age of Antibiotic Resistance: Evolution, Mechanisms, and Environmental Implications: A Review.Microorganisms · 2024Review
- Review
- Applications of different forms of nitrogen fertilizers affect soil bacterial community but not core ARGs profile.Frontiers in microbiology · 2024Article
- Chicken manure application alters microbial community structure and the distribution of antibiotic-resistance genes in rhizosphere soil of Cinnamomum camphora forests.FEMS microbiology ecology · 2023Article
- Contribution of Manure-Spreading Operations to Bioaerosols and Antibiotic Resistance Genes' Emission.Microorganisms · 2023Article
- Digestate from Agricultural Biogas Plants as a Reservoir of Antimicrobials and Antibiotic Resistance Genes-Implications for the Environment.International journal of environmental research and public health · 2023Article
- Diversity of antibiotic resistance genes in soils with four different fertilization treatments.Frontiers in microbiology · 2023Article
- Impact of the microbiome on human, animal, and environmental health from a One Health perspective.Science in One Health · 2023Review
- Study of the utilization of main crop straw resources in Southern China and its potential as a replacement for chemical fertilizers.Frontiers in plant science · 2023Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Manure, which contains large amounts of antibiotics and antibiotic resistance genes (ARGs), is widely used in agricultural soils and may lead to the evolution and dispersal of ARGs in the soil environment. In the present study, soils that received manure or chemical fertilizers for 15 years were sampled on the North China Plain (NCP), which is one of the primary areas of intensive agriculture in China. High-throughput quantitative PCR and sequencing technologies were employed to assess the effects of long-term manure or chemical fertilizer application on the distribution of ARGs and microbial communities. A total of 114 unique ARGs were successfully amplified from all soil samples. Manure application markedly increased the relative abundance and detectable numbers of ARGs, with up to 0.23 copies/16S rRNA gene and 81 unique ARGs. The increased abundance of ARGs in manure-fertilized soil was mainly due to the manure increasing the abundance of indigenous soil ARGs. In contrast, chemical fertilizers only moderately affected the diversity of ARGs and had no significant effect on the relative abundance of the total ARGs. In addition, manure application increased the abundance of mobile genetic elements (MGEs), which were significantly and positively correlated with most types of ARGs, indicating that horizontal gene transfer via MGEs may play an important role in the spread of ARGs. Furthermore, the application of manure and chemical fertilizers significantly affected microbial community structure, and variation partitioning analysis showed that microbial community shifts represented the major driver shaping the antibiotic resistome. Taken together, our results provide insight into the long-term effects of manure and chemical fertilization on the dissemination of ARGs in intensive agricultural ecosystems.
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