ArticleFrontiers in synaptic neuroscience2020
Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations.
Article in Frontiers in synaptic neuroscience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Article
- TRACR: an anterograde transneuronal tracing system for genetic access across synapses and longitudinal circuit analysis.bioRxiv : the preprint server for biology · 2026Article
- A Tac1-Expressing Brainstem Pathway Underlies the Pathogenesis of Trigeminal Neuralgia.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Aberrantly integrated adult-born immature neurons disrupt brain-wide networks during spatial memory processing.Molecular psychiatry · 2026Article
- Development and application of a barcoded rabies viral tracing method for mapping brain-wide inputs to single neurons.Cell reports methods · 2025Article
- Neural connectivity of oxytocin receptor-expressing neurons in the nucleus accumbens and their role in social attachment.Hormones and behavior · 2025Article
- Direct Hippocampal and Thalamic Inputs to Layer 3 Pyramidal Cells in the Medial Entorhinal Cortex Revealed by Monosynaptic Rabies Tracing.Neuroscience bulletin · 2025Article
- Layer-Specific Connectivity and Functional Interference of Chrna2+ Layer 5 Martinotti Cells in the Primary Motor Cortex.The European journal of neuroscience · 2025Article
- Complementary Organization of Mouse Driver and Modulator Cortico-thalamo-cortical Circuits.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Distinct structural and functional connectivity of genetically segregated thalamoreticular subnetworks.Cell reports · 2024Article
- Article
- 5' Transgenes drive leaky expression of 3' transgenes in Cre-inducible bi-cistronic vectors.Molecular therapy. Methods & clinical development · 2024Article
- Individual thalamic inhibitory interneurons are functionally specialized toward distinct visual features.Neuron · 2024Article
- Amylin Modulates a Ventral Tegmental Area-to-Medial Prefrontal Cortex Circuit to Suppress Food Intake and Impulsive Food-Directed Behavior.Biological psychiatry · 2024Article
- Diverse roles of pontine NPS-expressing neurons in sleep regulation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A flowchart for adequate controls in virus-based monosynaptic tracing experiments identified Cre-independent leakage of the TVA receptor in RΦGT mice.BMC neuroscience · 2024Article
- Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing.eLife · 2024Article
- Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses.Nature neuroscience · 2024Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus (RV) has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to a starting neuronal population. Its most common implementation is to use Cre mouse lines in combination with Cre-dependent "helper" adeno-associated viral vectors (AAVs) to supply the required genes to the targeted population before subsequent injection of a first-generation (ΔG) rabies viral vector. Here we show that the efficiency of transsynaptic spread and the degree of nonspecific labeling in wild-type control animals depend strongly on the concentrations of these helper AAVs. Our results suggest practical guidelines for achieving good results.
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