ArticleFrontiers in neural circuits2020
A Step-by-Step Protocol for Optogenetic Kindling.
Article in Frontiers in neural circuits, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Article
- Multi-Frequency Interpolation X-talk Removal Algorithm: Enabling Combinations of Concurrent Optogenetics and Lock-in Amplification Fiber Photometry via Removal of Optogenetic Stimulation Crosstalk.ACS chemical neuroscience · 2025Article
- Neurophotonic methods in approach to in vivo animal epileptic models: Advantages and limitations.Epilepsia · 2024Review
- Xenon LFP Analysis Platform Is a Novel Graphical User Interface for Analysis of Local Field Potential From Large-Scale MEA Recordings.Frontiers in neuroscience · 2022Article
- Therapeutic Investigation of Zingerone Against Pentylenetetrazole-Induced Kindled Seizures in Mice.Iranian journal of pharmaceutical research : IJPRArticle
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Authors and funding
2 authors.
Funding
Abstract
Electrical kindling, repeated brain stimulation eventually resulting in seizures, is widely used as an animal model of epileptogenesis and epilepsy. However, the stimulation electrode used for electric kindling targets unknown neuronal populations and may introduce tissue damage and inflammation. Optogenetics can be used to circumvent these shortcomings by permitting millisecond control of activity in genetically defined neurons without gross injury or inflammation. Here we describe an easy step-by-step protocol for optogenetic kindling - optokindling - by which seizures are eventually elicited in initially healthy mice through repeated light stimulation of neurons expressing Channelrhodopsin-2 (ChR2). Chronic EEG recordings may be performed over large time scales to monitor activity while video camera monitoring may be used to assess the behavioral severity of seizures. In conclusion, with optokindling, neuroscientists can elucidate the circuit changes that underpin epilepsy while minimizing the contribution of confounding factors such as brain damage and inflammation.
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