ArticleEnvironmental microbiology2020
Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota.
Article in Environmental microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
- Precision culturomics enabled by unlabeled single-cell morphology and Raman spectra.Nature communications · 2026Article
- RamEx: an R package for high-throughput microbial ramanome analyses with accurate quality assessment.Microbiome · 2026Article
- Persistent antimicrobial resistance during soil remediation driven by residual heavy metal co-selection.The ISME journal · 2026Article
- Rapid culture-free diagnosis of clinical pathogens via integrated microfluidic-Raman micro-spectroscopy.Nature communications · 2025Article
- Detecting and classifying metabolic activity ofBiosafety and health · 2025Article
- Soil warming increases the active antibiotic resistome in the gut of invasive giant African snails.Microbiome · 2025Article
- In situ degradation of 2-methylnaphthalene by a soil Penicillium strain associated with fungal-bacterial interactions.The ISME journal · 2025Article
- High-throughput single-cell sorting by stimulated Raman-activated cell ejection.Science advances · 2024Article
- Plasma-derived extracellular vesicles (EVs) as biomarkers of sepsis in burn patients via label-free Raman spectroscopy.Journal of extracellular vesicles · 2024Article
- Advances in single-cell sequencing technology in microbiome research.Genes & diseases · 2024Review
- Nanoplasmonic sensors for extracellular vesicles and bacterial membrane vesicles.Nano convergence · 2024Review
- Raman-Activated Cell Ejection for Validating the Reliability of the Raman Fingerprint Database of Foodborne Pathogens.Foods (Basel, Switzerland) · 2024Article
- Plasma-derived Extracellular Vesicles (EVs) as Biomarkers of Sepsis in Burn Patients via Label-free Raman Spectroscopy.bioRxiv : the preprint server for biology · 2024Article
- Microbial consortium assembly and functional analysis via isotope labelling and single-cell manipulation of polycyclic aromatic hydrocarbon degraders.The ISME journal · 2024Article
- Raman cell sorting for single-cell research.Frontiers in bioengineering and biotechnology · 2024Review
- High-throughput single-cell sorting by stimulated Raman-activated cell ejection.bioRxiv : the preprint server for biology · 2023Article
- Isolation and Cultivation of Human Gut Microorganisms: A Review.Microorganisms · 2023Review
- Metabolic Reprogramming in Colon Cancer Cells Persistently Infected with Newcastle Disease Virus.Cancers · 2023Article
- Active antibiotic resistome in soils unraveled by single-cell isotope probing and targeted metagenomics.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
The antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) in human gut microbiota have significant impact on human health. While high throughput metagenomic sequencing reveals genotypes of microbial communities, the functionality, phenotype and heterogeneity of human gut microbiota are still elusive. In this study, we applied Raman microscopy and deuterium isotope probing (Raman-DIP) to detect metabolic active ARB (MA-ARB) in situ at the single-cell level in human gut microbiota from two healthy adults. We analysed the relative abundances of MA-ARB under different concentrations of amoxicillin, cephalexin, tetracycline, florfenicol and vancomycin. To establish the link between phenotypes and genotypes of the MA-ARB, Raman-activated cell sorting (RACS) was used to sort MA-ARB from human gut microbiota, and mini-metagenomic DNA of the sorted bacteria was amplified, sequenced and analysed. The sorted MA-ARB and their associated ARGs were identified. Our results suggest a strong relation between ARB in human gut microbiota and personal medical history. This study demonstrates that the toolkit of Raman-DIP, RACS and DNA sequencing can be useful to unravel both phenotypes and genotypes of ARB in human gut microbiota at the single-cell level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.