Evidence map›Paper›PMID 32114386›Full record

ArticleEBioMedicine2020

Interactions between neutrophil extracellular traps and activated platelets enhance procoagulant activity in acute stroke patients with ICA occlusion.

Peng Zhou, Tao Li, Jiaqi Jin, Yingmiao Liu, Baorong Li, Quanye Sun, Jiawei Tian, Hongtao Zhao, Zhihui Liu, Shuai Ma and 4 more

Registry-linked trialOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EBioMedicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07354321 (Thrombo-inflammation Des Maladies Neurovascualires aiguës), which is not on this map. Cited by 96 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
96citing papers in PubMed, 3 pooled it
7.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT07354321 not yet recruitingnot on this mapstarted 2026, after this paper: background citation

Thrombo-inflammation Des Maladies Neurovascualires aiguës : définition de Nouvelles Cibles thérapeutiques (TARGETS)

TypeobservationalSponsorFrançois DelvoyeRan2026 to 2032Enrolled499ConditionsDelayed Cerebral Ischemia After Subarachnoid Hemorrhage, Ischemic Stroke of Large or Medium Vessels
3 · Its place in the literature

Who cites it

96 citing papers in PubMed, 3 syntheses or guidelines pooled it, 135 citations in OpenAlex.

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  15. Role of neutrophils in regulating vascular permeability in inflammatory and autoimmune diseases.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026
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36 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

Peng ZhouDepartment of Neurosurgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, PR China; The Key Laboratory of Myocardial Ischemia, Ministry of Education, Heilongjiang Province, Harbin, PR China.
Tao LiDepartment of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Jiaqi JinDepartment of Neurosurgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Yingmiao LiuDepartment of Stomatology, The First Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Baorong LiDepartment of Stomatology, The First Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Quanye SunDepartment of Clinical Laboratory, Qingdao Municipal Hospital Group, Qingdao, PR China.
Jiawei TianDepartment of Neurosurgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Hongtao ZhaoDepartment of Neurosurgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Zhihui LiuDepartment of Neurosurgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Shuai MaDepartment of Neurosurgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Shuoqi ZhangDepartment of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Valerie A NovakovicDepartment of Research, Brigham and Women's Hospital, VA Boston Healthcare System, Harvard Medical School, Boston, MA, USA.
Jialan ShiDepartment of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, PR China; Department of Surgery, Brigham and Women's Hospital, VA Boston Healthcare System, Harvard Medical School, Boston, MA, USA. Electronic address: jialan_shi@hms.harvard.edu.
Shaoshan HuDepartment of Neurosurgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, PR China. Electronic address: shaoshanhu421@126.com.
Harbin Medical University · CNSecond Affiliated Hospital of Harbin Medical University · CNVA Boston Healthcare System · USFirst Affiliated Hospital of Harbin Medical University · CNQingdao Municipal Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe role of neutrophil extracellular traps (NETs) in procoagulant activity (PCA) in stroke patients caused by thromboembolic occlusion of the internal carotid artery (ICA) remains unclear. Our objectives were to evaluate the critical role of NETs in the induction of hypercoagulability in stroke and to identify the functional significance of NETs during atherothrombosis.

methodsThe levels of NETs, activated platelets (PLTs), and PLT-derived microparticles (PMPs) were detected in the plasma of 55 stroke patients and 35 healthy controls. NET formation and thrombi were analysed using immunofluorescence. Exposed phosphatidylserine (PS) was evaluated with flow cytometry and confocal microscopy. PCA was analysed using purified coagulation complex, thrombin, and fibrin formation assays.

findingsThe plasma levels of NETs, activated PLTs, and PMP markers in the carotid lesion site (CLS) were significantly higher than those in the aortic blood. NETs were decorated with PS in thrombi and the CLS plasma of ICA occlusion patients. Notably, the complementary roles of CLS plasma and thrombin-activated PLTs were required for NET formation and subsequent PS exposure. PS-bearing NETs provided functional platforms for PMPs and coagulation factor deposition and thus increased thrombin and fibrin formation. DNase I and lactadherin markedly inhibited these effects. In addition, NETs were cytotoxic to endothelial cells, converting these cells to a procoagulant phenotype. Sivelestat, anti-MMP9 antibody, and activated protein C (APC) blocked this cytotoxicity by 25%, 39%, or 52%, respectively.

interpretationNETs played a pivotal role in the hypercoagulability of stroke patients. Strategies that prevent NET formation may offer a potential therapeutic strategy for thromboembolism interventions.

fundingThis study was supported by grants from the National Natural Science Foundation of China (61575058, 81873433 and 81670128) and Graduate Innovation Fund of Harbin Medical University (YJSKYCX2018-58HYD).

Indexed as

Blood CoagulationAdolescentAdultAgedAged, 80 and overBlood PlateletsCarotid Artery, InternalCarotid Artery ThrombosisCell-Derived MicroparticlesExtracellular TrapsFemaleFibrinGlycineHumansHuman Umbilical Vein Endothelial CellsMaleFibrinGlycinePhosphatidylserinessivelestatSulfonamidesThrombinEndothelial cellsHypercoagulable stateNeutrophil extracellular trapsPhosphotidylserineStrokeThrombosis

Identifiers

PMID32114386
PMCPMC7047181
OpenAlexW3008363131

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.