ArticleGastroenterology2020
Robust, 3-Dimensional Visualization of Human Colon Enteric Nervous System Without Tissue Sectioning.
Article in Gastroenterology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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Who cites it
48 citing papers in PubMed, 64 citations in OpenAlex.
- Subtypes of Enteric Neurons in Hirschsprung Disease: A Comparison of Fully Ganglionic and Transitional Zone.Neurogastroenterology and motility · 2026Article
- Full thickness three-dimensional mapping of neuronal populations within the avian enteric nervous system.Poultry science · 2026Article
- Three-dimensional modeling of sensory nerve architecture and eosinophil and mast cell interactions in eosinophilic gastrointestinal disease.Journal of leukocyte biology · 2026Article
- enGLOW 3D microscopy of the enteric nervous system in cleared human and mouse gut.Communications biology · 2026Article
- Enteric nervous system degeneration in human and murine CLN3 disease, is ameliorated by gene therapy in mice.Acta neuropathologica communications · 2025Article
- Stereoscopic Quantitative Analysis of Enteric Nervous System in Patients With Slow Transit Constipation.Neurogastroenterology and motility · 2025Article
- Intramuscular enteric glia persist in Hirschsprung disease and undergo neurogenesis in response to GDNF-NCAM1 signaling.Scientific reports · 2025Article
- Scientific evolution from the definition of Hirschsprung disease to the present: a bibliometric analysis (1980-2023).Pediatric research · 2025Article
- Three-dimensional imaging and computational quantitation as a novel approach to assess nerve fibers, enteric glial cells, mast cells, and the proximity of mast cells to the nerve fibers in human sigmoid mucosal biopsies from healthy subjects.Journal of neuroscience methods · 2025Article
- Flow Cytometric Analysis and Sorting of Murine Enteric Nervous System Cells: An Optimized Protocol.International journal of molecular sciences · 2025Article
- The Neuroimmune Axis in Gastrointestinal Disorders - An Underrecognized Problem.Current gastroenterology reports · 2025Review
- Gene therapy ameliorates bowel dysmotility and enteric neuron degeneration and extends survival in lysosomal storage disorder mouse models.Science translational medicine · 2025Article
- Imaging the enteric nervous system.Frontiers in neuroanatomy · 2025Review
- AI-powered 3D pathology protocol enhances enteric nervous system visualization and quantification for clinical diagnostics.Theranostics · 2025Article
- Current and future state of the management of Hirschsprung disease.World journal of pediatric surgery · 2025Review
- Optimization of protocols for immunohistochemical assessment of enteric nervous system in formalin fixed human tissue.bioRxiv : the preprint server for biology · 2024Article
- Gut Analysis Toolbox - automating quantitative analysis of enteric neurons.Journal of cell science · 2024Article
- Volumetric changes of the enteric nervous system under physiological and pathological conditions measured using x-ray phase-contrast tomography.JGH open : an open access journal of gastroenterology and hepatology · 2024Article
- Neuregulin 1 (NRG1) and its receptors in the enteric nervous system and other parts of the gastrointestinal wall.Histology and histopathology · 2024Review
- Three-Dimensional Imaging of the Enteric Nervous System in Human Pediatric Colon Reveals New Features of Hirschsprung's Disease.Gastroenterology · 2024Article
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 1 country.
Funding
Abstract
BACKGROUND &
aimsSmall, 2-dimensional sections routinely used for human pathology analysis provide limited information about bowel innervation. We developed a technique to image human enteric nervous system (ENS) and other intramural cells in 3 dimensions.
methodsUsing mouse and human colon tissues, we developed a method that combines tissue clearing, immunohistochemistry, confocal microscopy, and quantitative analysis of full-thickness bowel without sectioning to quantify ENS and other intramural cells in 3 dimensions.
resultsWe provided 280 adult human colon confocal Z-stacks from persons without known bowel motility disorders. Most of our images were of myenteric ganglia, captured using a 20× objective lens. Full-thickness colon images, viewed with a 10× objective lens, were as large as 4 × 5 mm
conclusionsWe developed a 3-dimensional imaging method for colon that provides more information about ENS structure than tissue sectioning. This approach could improve diagnosis for human bowel motility disorders and may be useful for other bowel diseases as well.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.