Evidence map›Paper›PMID 32112874›Full record

ArticleThe ocular surface2020

Dihydrotestosterone suppression of proinflammatory gene expression in human meibomian gland epithelial cells.

Afsun Sahin, Yang Liu, Wendy R Kam, Raheleh Rahimi Darabad, David A Sullivan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The ocular surface, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Meibomian Gland Dysfunction Clinical Practice Guidelines.Japanese journal of ophthalmology · 2023
    Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. The cornea in keratoconjunctivitis sicca.Experimental eye research · 2020
    Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Afsun SahinSchepens Eye Research Institute of Massachusetts Eye and Ear and Department of Ophthalmology, Harvard Medical School, Boston, MA, USA; Department of Ophthalmology, Koc University Medical School, Istanbul, Turkey.
Yang LiuSchepens Eye Research Institute of Massachusetts Eye and Ear and Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.
Wendy R KamSchepens Eye Research Institute of Massachusetts Eye and Ear and Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.
Raheleh Rahimi DarabadSchepens Eye Research Institute of Massachusetts Eye and Ear and Department of Ophthalmology, Harvard Medical School, Boston, MA, USA; Department of Clinical Anesthesia, Indiana University School of Medicine, Indianapolis, IN, USA.
David A SullivanSchepens Eye Research Institute of Massachusetts Eye and Ear and Department of Ophthalmology, Harvard Medical School, Boston, MA, USA. Electronic address: david_sullivan@meei.harvard.edu.
Massachusetts Eye and Ear Infirmary · US

Funding

Sex, sex steroids and dry eye syndromesR01EY005612 · NEI · SCHEPENS EYE RESEARCH INSTITUTE · PI SULLIVAN, DAVID A. · 1985 to 2014
$9.0M
NEI NIH HHS R01 EY005612
6 · The paper itself

Abstract

purposeWe discovered that dihydrotestosterone (DHT) decreases the ability of lipopolysaccharide, a bacterial toxin, to stimulate the secretion of leukotriene B4, a potent proinflammatory mediator, by immortalized human meibomian gland epithelial cells (IHMGECs). We hypothesize that this hormone action reflects an androgen suppression of proinflammatory gene activity in these cells. Our goal was to test this hypothesis. For comparison, we also examined whether DHT treatment elicits the same effect in immortalized human corneal (IHC) and conjunctival (IHConj) ECs.

methodsDifferentiated cells were cultured in media containing vehicle or 10 nM DHT. Cells (n = 3 wells/treatment group) were then processed for RNA isolation and the analysis of gene expression by using Illumina BeadChips, background subtraction, cubic spline normalization and Geospiza software.

resultsOur results demonstrate that DHT significantly suppressed the expression of numerous immune-related genes in HMGECs, such as those associated with antigen processing and presentation, innate and adaptive immune responses, chemotaxis, and cytokine production. DHT also enhanced the expression of genes for defensin β1, IL-1 receptor antagonist, and the anti-inflammatory serine peptidase inhibitor, Kazal type 5. In contrast, DHT had no effect on proinflammatory gene expression in HCECs, and significantly increased 33 gene ontologies linked to the immune system in HConjECs.

conclusionsOur findings support our hypothesis that androgens suppress proinflammatory gene expression in IHMGECs. This hormone effect may contribute to the typical absence of inflammation within the human meibomian gland.

Indexed as

Meibomian GlandsCells, CulturedDihydrotestosteroneEpithelial CellsGene ExpressionHumansDihydrotestosteroneAndrogenConjunctivaCorneaDihydrotestosteroneEpithelial cellsGene expressionHumanInflammationMeibomian gland

Identifiers

PMID32112874
PMCPMC7195874
OpenAlexW3008437295

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.