Evidence map›Paper›PMID 32112374›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Using Bioengineered Bioluminescence to Track Stem Cell Transplantation In Vivo.

Dong Han, Joseph C Wu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Evaluation of differentScientific reports · 2022
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Dong HanStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA.
Joseph C WuStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA. joewu@stanford.edu.
Cardiovascular Institute of the South · USStanford University · US

Funding

Single Cell Sequencing of Human iPSC-CM Subtype Identity and FunctionR01HL145676 · NHLBI · STANFORD UNIVERSITY · PI MARK MERCOLA, Joseph C. Wu · 2019 to 2026
$5.1M
Molecular Imaging of Cardiac Pluripotent Stem CellsR01HL133272 · NHLBI · STANFORD UNIVERSITY · PI WU, JOSEPH C. · 2016 to 2020
$2.4M
Assessment of Low-Dose Radiation Risk and Mechanisms of Individual RadiosensitivityR01HL132875 · NHLBI · STANFORD UNIVERSITY · PI WU, JOSEPH C. · 2016 to 2019
$1.9M
NHLBI NIH HHS R01 HL132875NHLBI NIH HHS R01 HL133272NHLBI NIH HHS R01 HL145676
6 · The paper itself

Abstract

Bioluminescence imaging enables the real-time detection and tracking of engrafted cells in vivo noninvasively and dynamically. By detecting and quantifying the photons released from the oxidation of luciferin catalyzed by luciferase enzymes, this approach has proven effective in tracking engrafted stem cell survival and retention, making it a powerful tool to monitor cell fate after transplantation without animal sacrifice. Here we describe a protocol that allows luciferase-labeled stem cells to be imaged and tracked in vivo by bioluminescent imaging via an IVIS spectrum imaging system.

Indexed as

AnimalsCell DifferentiationCell TrackingGenes, ReporterLuciferases, FireflyLuminescent MeasurementsStem CellsStem Cell TransplantationLuciferases, FireflyBioluminescenceCell trackingLuciferaseMolecular imaging

Identifiers

PMID32112374
PMCPMC10902212
OpenAlexW3006753971

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.