ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2020
A R2R3-MYB gene-based marker for the non-darkening seed coat trait in pinto and cranberry beans (Phaseolus vulgaris L.) derived from 'Wit-rood boontje'.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 22 citations in OpenAlex.
- Genomic resources to advance seed coat color and patterning genetics and breeding in common bean (Phaseolus vulgaris L.).The plant genome · 2026Article
- Molecular insights into postharvest seed coat darkening in common beans: a look beyond theFrontiers in plant science · 2025Article
- Proanthocyanidin biosynthesis and postharvest seed coat darkening in pinto bean.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2025Review
- Sensory properties of selected biofortified common bean (Food science & nutrition · 2024Article
- Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis.Horticulture research · 2024Article
- Genomic insights into genetic diversity and seed coat color change in common bean composite populations.Frontiers in plant science · 2024Article
- Genome-Wide Association Study to Identify Possible Candidate Genes of Snap Bean Leaf and Pod Color.Genes · 2023Article
- Monitoring Stimulated Darkening from UV-C Light on Different Bean Genotypes by NMR Spectroscopy.Molecules (Basel, Switzerland) · 2022Article
- Global analysis of common bean multidrug and toxic compound extrusion transporters (PvMATEs): PvMATE8 and pinto bean seed coat darkening.Frontiers in plant science · 2022Article
- Genome-wide association mapping reveals new loci associated with light-colored seed coat at harvest and slow darkening in carioca beans.BMC plant biology · 2021Article
- Genetic Mapping and Identification of the Candidate Gene for White Seed Coat inInternational journal of molecular sciences · 2021Article
- QTL Mapping of Seed Quality Traits Including Cooking Time, Flavor, and Texture in a Yellow Dry Bean (Frontiers in plant science · 2021Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
key messageThe gene Phvul.010G130600 which codes for a MYB was shown to be tightly associated with seed coat darkening in Phaseolus vulgaris and a single nucleotide deletion in the allele in Wit-rood disrupts a transcription activation region that likely prevents its functioning in this non-darkening genotype. The beige and white background colors of the seed coats of conventional pinto and cranberry beans turn brown through a process known as postharvest darkening (PHD). Seed coat PHD is attributed to proanthocyanidin accumulation and its subsequent oxidation in the seed coat. The J gene is an uncharacterized classical genetic locus known to be responsible for PHD in common bean (P. vulgaris) and individuals that are homozygous for its recessive allele have a non-darkening (ND) seed coat phenotype. A previous study identified a major colorimetrically determined QTL for seed coat color on chromosome 10 that was associated with the ND trait. The objectives of this study were to identify a gene associated with seed coat postharvest darkening in common bean and understand its function in promoting seed coat darkening. Amplicon sequencing of 21 candidate genes underlying the QTL associated with the ND trait revealed a single nucleotide deletion (c.703delG) in the candidate gene Phvul.010G130600 in non-darkening recombinant inbred lines derived from crosses between ND 'Wit-rood boontje' and a regular darkening pinto genotype. In silico analysis indicated that Phvul.010G130600 encodes a protein with strong amino acid sequence identity (70%) with a R2R3-MYB-type transcription factor MtPAR, which has been shown to regulate proanthocyanidin biosynthesis in Medicago truncatula seed coat tissue. The deletion in the 'Wit-rood boontje' allele of Phvul.010G130600 likely causes a translational frame shift that disrupts the function of a transcriptional activation domain contained in the C-terminus of the R2R3-MYB. A gene-based dominant marker was developed for the dominant allele of Phvul.010G130600 which can be used for marker-assisted selection of ND beans.
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