ReviewNucleic acids research2020
Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors.
Review in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 178 citations in OpenAlex.
- Structural Basis and Conformational Switching of Capture-SELEX Aptamers for Designing Equol Sensors.ACS omega · 2026Article
- Rational Design Strategies for Stimuli-Responsive DNAzymes Using Modified and Artificial Nucleotides.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Methodological Landscape of DNA Damage Response Detection: From Conventional Assays to Future Innovations.Current issues in molecular biology · 2026Review
- Controlled Reassociation of Multistranded, Polycrossover DNA Molecules into Double Helices.Nano letters · 2025Article
- From liquid-phase synthesis to chemical ligation: preparation of oligonucleotides and their backbone analogs in solution.Nucleic acids research · 2025Review
- Programmable DNA aptamer logic gates: from structural design to integrated systems for intelligent nanoscale biosensors.Analytical and bioanalytical chemistry · 2025Review
- Cation-Controlled Assembly, Activity, and Organization of Biomimetic DNA Receptors in Synthetic Cell Membranes.Journal of the American Chemical Society · 2025Article
- Advancements and Applications of Split Technology in CRISPR/Cas12a: Transforming Molecular Diagnostics and Biosensing.Biosensors · 2025Review
- Recent Advances in Functionally Engineered Aptamers: Strategies and Applications.Topics in current chemistry (Cham) · 2025Review
- Aptamer Sequence Optimization and Its Application in Food Safety Analysis.Foods (Basel, Switzerland) · 2025Review
- Ribozyme-Catalyzed Site-Specific Labeling of RNA Using OAngewandte Chemie (International ed. in English) · 2025Article
- Quantity of Cu(II) ions in a copper pot by a DNAzyme-based fluorescent sensor.Food chemistry. Molecular sciences · 2025Article
- Review
- Rapid discovery of functional RNA domains.Nucleic acids research · 2025Article
- A microscale thermophoresis-based enzymatic RNA methyltransferase assay enables the discovery of DNMT2 inhibitors.Communications chemistry · 2025Article
- Proximity-activated guide RNA of CRISPR-Cas12a for programmable diagnostic detection and gene regulation.Nucleic acids research · 2025Article
- Genetically Encoded Fluorogenic DNA Aptamers for Imaging Metabolite in Living Cells.Journal of the American Chemical Society · 2025Article
- Modular DNA origami-based electrochemical detection of DNA and proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Recent advances in aptamer discovery, modification and improving performance.Biochemistry and biophysics reports · 2024Review
- Adaptation of a Model Spike Aptamer for Isothermal Amplification-Based Sensing.Sensors (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In analogy to split-protein systems, which rely on the appropriate fragmentation of protein domains, split aptamers made of two or more short nucleic acid strands have emerged as novel tools in biosensor set-ups. The concept relies on dissecting an aptamer into a series of two or more independent fragments, able to assemble in the presence of a specific target. The stability of the assembled structure can further be enhanced by functionalities that upon folding would lead to covalent end-joining of the fragments. To date, only a few aptamers have been split successfully, and application of split aptamers in biosensing approaches remains as promising as it is challenging. Further improving the stability of split aptamer target complexes and with that the sensitivity as well as efficient working modes are important tasks. Here we review functional nucleic acid assemblies that are derived from aptamers and ribozymes/DNAzymes. We focus on the thrombin, the adenosine/ATP and the cocaine split aptamers as the three most studied DNA split systems and on split DNAzyme assemblies. Furthermore, we extend the subject into split light up RNA aptamers used as mimics of the green fluorescent protein (GFP), and split ribozymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.