ArticleNature communications2020
In vitro ribosome synthesis and evolution through ribosome display.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Ribosome engineering enhances genetic code expansion inSynthetic and systems biotechnology · 2027Article
- Peptide Aptamers: Innovative Design and Applications in Pathogen Detection.Chembiochem : a European journal of chemical biology · 2026Review
- In Vitro Selection of Antibodies by Ribosome Display.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Hydrogel particle-based protein display enabled by particle-templated emulsification.RSC advances · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Autonomous ribosome biogenesis in vitro.Nature communications · 2025Article
- Reaching New Heights in Genetic Code Manipulation with High Throughput Screening.Chemical reviews · 2024Review
- An Automated Cell-Free Workflow for Transcription Factor Engineering.ACS synthetic biology · 2024Article
- Encoding and display technologies for combinatorial libraries in drug discovery: The coming of age from biology to therapy.Acta pharmaceutica Sinica. B · 2024Review
- Alternate conformational trajectories in ribosome translocation.PLoS computational biology · 2024Article
- Design of Novel Synthetic RNA Replicons Based onACS synthetic biology · 2024Article
- Antibody design using deep learning: from sequence and structure design to affinity maturation.Briefings in bioinformatics · 2024Review
- Critical steps in the assembly process of the bacterial 50S ribosomal subunit.Nucleic acids research · 2024Review
- Ribosome Pool Engineering Increases Protein Biosynthesis Yields.ACS central science · 2024Article
- Building Synthetic Cells─From the Technology Infrastructure to Cellular Entities.ACS synthetic biology · 2024Review
- Translational impacts of enzymes that modify ribosomal RNA around the peptidyl transferase centre.RNA biology · 2024Article
- Emerging Quantitative Biochemical, Structural, and Biophysical Methods for Studying Ribosome and Protein-RNA Complex Assembly.Biomolecules · 2023Review
- Near-physiological in vitro assembly of 50S ribosomes involves parallel pathways.Nucleic acids research · 2023Article
- Community science designed ribosomes with beneficial phenotypes.Nature communications · 2023Article
- Cell-free Biosynthesis of Peptidomimetics.Biotechnology and bioprocess engineering : BBE · 2023Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Directed evolution of the ribosome for expanded substrate incorporation and novel functions is challenging because the requirement of cell viability limits the mutations that can be made. Here we address this challenge by combining cell-free synthesis and assembly of translationally competent ribosomes with ribosome display to develop a fully in vitro methodology for ribosome synthesis and evolution (called RISE). We validate the RISE method by selecting active genotypes from a ~1.7 × 10
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