Evidence map›Paper›PMID 32111746›Full record

ArticleIn vivo (Athens, Greece)

Effect of Physical Training on the Levels of Sex Hormones and the Expression of Their Receptors in Rats With Induced Mammary Cancer in Secondary Prevention Model - Preliminary Study.

Katarzyna Siewierska, Iwona Malicka, Christopher Kobierzycki, Jedrzej Grzegrzolka, Aleksandra Piotrowska, Urszula Paslawska, Marek Cegielski, Marzenna Podhorska-Okolow, Piotr Dziegiel, Marek Wozniewski

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Katarzyna SiewierskaDepartment of Physiotherapy, University School of Physical Education, Wroclaw, Poland.
Iwona MalickaDepartment of Physiotherapy, University School of Physical Education, Wroclaw, Poland.
Christopher KobierzyckiDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland christopher.kobierzycki@umed.wroc.pl.
Jedrzej GrzegrzolkaDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland.
Aleksandra PiotrowskaDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland.
Urszula PaslawskaDepartment of Internal Medicine and Clinic of Diseases of Horses, Dogs, and Cats, University of Environmental and Life Sciences, Wroclaw, Poland.
Marek CegielskiDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland.
Marzenna Podhorska-OkolowDivision of Ultrastructure Research, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland.
Piotr DziegielDepartment of Physiotherapy, University School of Physical Education, Wroclaw, Poland.
Marek WozniewskiDepartment of Physiotherapy, University School of Physical Education, Wroclaw, Poland.
Wroclaw Medical University · PLUniversity School of Physical Education in Wroclaw · PLWroclaw University of Environmental and Life Sciences · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

aimBreast cancer is the most common malignant tumor among women worldwide. In previous work, we presented results of physical activity in primary prevention in a model of induced mammary gland cancer. In the present study, we assessed the influence of physical activity on sex hormone levels (estradiol and progesterone) and the expression of their receptors (ER, PR), as well as the level of apoptosis of tumor cells in secondary prevention. MATERIALS AND

methodsFifty 1-month-old female Sprague-Dawley rats received intraperitoneal injection of 180 mg/kg body weight of N-methyl-N-nitrosourea (MNU) for tumor induction. Three months after the administration of MNU, rats were divided into four groups: low-intensity, moderate-intensity, and high-intensity physical training groups (combined as PT) and a sedentary control (SC) group. Physical training was conducted 5 days per week with a three-position treadmill according to a precisely described protocol. The entire training was completed by 32 rats from which tissue and blood were collected for further analysis. Immunohistochemistry for ER and PR expression, terminal deoxynucleotidyl transferase dUTP nick-end labeling method for detection of apoptosis, and enzyme-linked fluorescent assay for detection of plasma hormone levels (estradiol and progesterone) were performed. Statistical analysis used p<0.05 as the significance level.

resultsSignificantly stronger expression of ER and PR was found in the SC in comparison to the PT group (p=0.035 and p=0.036, respectively). No statistically significant differences were found in estradiol or progesterone concentrations between SC and PT groups. Apoptosis was non-significantly increased in the PT group in comparison with the SC group. Stronger apoptosis in the PT group correlated positively with the level of training intensity (r=0.35, p=0.05).

conclusionPhysical training may reduce ER and PR expression in breast cancer cells, and reduce cell sensitivity to pro-proliferative and anti-apoptotic effects of estrogens, ultimately leading to apoptosis.

Indexed as

Physical Conditioning, AnimalSecondary PreventionAnimalsDisease Models, AnimalFemaleGene ExpressionGonadal Steroid HormonesImmunohistochemistryMammary Neoplasms, ExperimentalRatsReceptors, EstrogenReceptors, ProgesteroneReceptors, SteroidGonadal Steroid HormonesReceptors, EstrogenReceptors, ProgesteroneReceptors, Steroidbreast cancerestradiolEstrogen receptorN-methyl-N-nitrosoureaphysical activityprogesteroneprogesterone receptorrat experimental model

Identifiers

PMID32111746
PMCPMC7157892
OpenAlexW3007772641

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.