Evidence map›Paper›PMID 32111730›Full record

ArticleJournal for immunotherapy of cancer2020

Novel, genetically induced mouse model that recapitulates the histological morphology and immunosuppressive tumor microenvironment of metastatic peritoneal carcinomatosis.

Ssu-Hsueh Tseng, Sung-Taek Park, Brandon Lam, Ya-Chea Tsai, Max A Cheng, Emily Farmer, Deyin Xing, Chien-Fu Hung

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal for immunotherapy of cancer, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Trial
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Ssu-Hsueh TsengPathology, Johns Hopkins University, Baltimore, Maryland, USA.
Sung-Taek ParkPathology, Johns Hopkins University, Baltimore, Maryland, USA.
Brandon LamPathology, Johns Hopkins University, Baltimore, Maryland, USA.
Ya-Chea TsaiPathology, Johns Hopkins University, Baltimore, Maryland, USA.
Max A ChengPathology, Johns Hopkins University, Baltimore, Maryland, USA.
Emily FarmerPathology, Johns Hopkins University, Baltimore, Maryland, USA.
Deyin Xing *Pathology, Johns Hopkins University, Baltimore, Maryland, USA chung2@jhmi.edu dxing2@jhmi.edu.
Chien-Fu Hung *Pathology, Johns Hopkins University, Baltimore, Maryland, USA chung2@jhmi.edu dxing2@jhmi.edu.
Johns Hopkins University · US

Funding

Mouse modeling of HPV infectionR01CA233486 · NCI · JOHNS HOPKINS UNIVERSITY · PI HUNG, CHIEN-FU, RODEN, RICHARD BRUCE · 2019 to 2023
$3.0M
Development of Novel Spontaneous HPV Cervicovaginal Carcinoma Models for Cancer ImmunotherapyR01CA237067 · NCI · JOHNS HOPKINS UNIVERSITY · PI WU, TZYY-CHOOU · 2019 to 2023
$2.9M
The role of Albumin-Flt3L-induced cross-presenting dendritic cell expansion in antitumor immunityF31CA236051 · NCI · JOHNS HOPKINS UNIVERSITY · PI LAM, BRANDON KRISHNA · 2019 to 2021
$128k
NCI NIH HHS F31 CA236051NCI NIH HHS R01 CA233486NCI NIH HHS R01 CA237067
6 · The paper itself

Abstract

backgroundPeritoneal carcinomatosis is a hallmark of advanced peritoneal tumor progression, particularly for tubal/ovarian high-grade serous carcinomas (HGSCs). Patients with peritoneal carcinomatosis have poor survival rates and are difficult to treat clinically due to widespread tumor dissemination in the peritoneal cavity.

methodsWe developed a clinically relevant, genetically induced, peritoneal carcinomatosis model that recapitulates the histological morphology and immunosuppressive state of the tumor microenvironment of metastatic peritoneal HGSCs by intraperitoneally injecting shp53, AKT, c-Myc, luciferase and sleeping beauty transposase, followed by electroporation (EP) in the peritoneal cavity of immunocompetent mice (intraperitoneal (IP)/EP mice).

resultsSimilar to the spread of human ovarian cancers, IP/EP mice displayed multiple tumor nodules attached to the surface of the abdomen. Histopathological analysis indicated that these tumors were epithelial in origin. These IP/EP mice also displayed a loss of CD3

conclusionsOverall, our tumor model recapitulates clinical peritoneal HGSC metastasis, which makes it ideal for preclinical drug screening, testing of immunotherapy-based therapeutics and studying of the tumor biology of peritoneal carcinomatosis.

Indexed as

AnimalsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell Line, TumorDisease Models, AnimalElectroporationFemaleHumansLymphocytes, Tumor-InfiltratingMiceMice, TransgenicOncogenesOvarian NeoplasmsPeritoneal NeoplasmsPrimary Cell CultureTransposasesTransposasesTrp53 protein, mouseTumor Suppressor Protein p53immunotherapytranslational medical researchvaccination

Identifiers

PMID32111730
PMCPMC7057437
OpenAlexW3007195872

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.