ArticleParasites & vectors2020
Probe-based multiplex qPCR identifies blood-meal hosts in Anopheles mosquitoes from Papua New Guinea.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Bloodmeal metabarcoding reveals host feeding patterns for Aedes aegypti and Culex quinquefasciatus in Jutiapa, Guatemala and Texas, USA.Scientific reports · 2026Article
- Bloodmeal metabarcoding reveals host feeding patterns for Aedes aegypti and Culex quinquefasciatus in Jutiapa, Guatemala and Texas, USA.Research square · 2026Article
- ElevatedInsects · 2025Article
- À la carte: how mosquitoes choose their blood meals.Trends in parasitology · 2024Review
- Article
- Genotyping of Anopheles mosquito blood meals reveals nonrandom human host selection: implications for human-to-mosquito Plasmodium falciparum transmission.Malaria journal · 2023Article
- Blood-feeding patterns of Anopheles vectors of human malaria in Malawi: implications for malaria transmission and effectiveness of LLIN interventions.Malaria journal · 2022Article
- Vector composition, abundance, biting patterns and malaria transmission intensity in Madang, Papua New Guinea: assessment after 7 years of an LLIN-based malaria control programme.Malaria journal · 2022Article
- Flavivirus Persistence in Wildlife Populations.Viruses · 2021Review
- Nonrandom Selection and Multiple Blood Feeding of Human Hosts by Anopheles Vectors: Implications for Malaria Transmission in Papua New Guinea.The American journal of tropical medicine and hygiene · 2021Article
- Blood meal source and mixed blood-feeding influence gut bacterial community composition in Aedes aegypti.Parasites & vectors · 2021Article
- Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundDetermination of blood-meal hosts in blood-fed female Anopheles mosquitoes is important for evaluating vectorial capacity of vector populations and assessing effectiveness of vector control measures. Sensitive molecular methods are needed to detect traces of host blood in mosquito samples, to differentiate hosts, and to detect mixed host blood meals. This paper describes a molecular probe-based quantitative PCR for identifying blood-meal hosts in Anopheles malaria vectors from Papua New Guinea.
methodsTaqMan oligonucleotide probes targeting specific regions of mitochondrial or nuclear DNA of the three primary Anopheles blood-meal hosts, humans, pigs and dogs, were incorporated into a multiplex, quantitative PCR which was optimized for sensitivity and specificity.
resultsAmplification of serially diluted DNA showed that the quantitative PCR detected as low as 10
conclusionsThe multiplex quantitative PCR described here is sensitive at detecting low DNA concentration and mixed host DNA in samples and useful for blood-meal analysis of field mosquitoes, in particular mixed-host blood meals.
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