ArticleBMC molecular and cell biology2020
Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts.
Article in BMC molecular and cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Integrated Size-Selective Cell Purification and Electroporation for Genetic Manipulation of Primary Cells.Micromachines · 2026Article
- Electroporation induced changes in extracellular vesicle profile.Drug delivery · 2025Article
- Effect of degeneration stage on non-viral tissue transfection ofMolecular therapy. Nucleic acids · 2025Article
- High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds.Journal of visualized experiments : JoVE · 2025Article
- Tissue nanotransfection and cellular reprogramming in regenerative medicine and antimicrobial dynamics.Frontiers in bioengineering and biotechnology · 2025Review
- Enhancement effect of urea toward electroporation-mediated plasmid transfection efficiency in the HEK-293 cell line.Research in pharmaceutical sciences · 2024Article
- Dynamic maternal synthesis and segregation of the germ plasm organizer, Bucky ball, in chicken oocytes and follicles.Scientific reports · 2024Article
- Condition optimization for electroporation transfection in horse skeletal muscle satellite cells.Animal biotechnology · 2024Article
- A solution for highly efficient electroporation of primary cytotoxic T lymphocytes.BMC biotechnology · 2024Article
- Stability of polymeric cationic niosomes and their plasmid DNA-based complexes as gene delivery carriers.Drug delivery · 2023Article
- Optimization of piggyBac Transposon System Electrotransfection in Sheep Fibroblasts.Molecular biotechnology · 2023Article
- Scalable continuous-flow electroporation platform enabling T cell transfection for cellular therapy manufacturing.Scientific reports · 2023Article
- Upregulation of host genes during disease progression in bovine leukemia virus infection is independent of overexpression of viral transcriptional regulators in vitro.Archives of virology · 2023Article
- CRISPR/Cas9-mediated targeted knock-in of large constructs using nocodazole and RNase HII.Scientific reports · 2023Article
- Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis.Pharmaceutics · 2022Review
- Systemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model.Biomedicines · 2022Article
- Application of transposon systems in the transgenesis of bovine somatic and germ cells.BMC veterinary research · 2022Article
- Gene delivery in adherent and suspension cells using the combined physical methods.Cytotechnology · 2022Article
- Ultralong-Time Recovery and Low-Voltage Electroporation for Biological Cell Monitoring Enabled by a Microsized Multipulse Framework.ACS omega · 2021Article
- Fabrication and use of silicon hollow-needle arrays to achieve tissue nanotransfection in mouse tissue in vivo.Nature protocols · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundGene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than immortalized cells, and have a limited proliferative capacity. Improving the gene transfer by using square wave electroporation in difficult to transfect cells, like bovine fetal fibroblasts, is a prerequisite for transgenic and further downstream experiments.
resultsHere, bovine fetal fibroblasts were used for square-wave electroporation experiments in which the following parameters were systematically tested: electroporation buffer, electroporation temperature, pulse voltage, pulse duration, pulse number, cuvette type and plasmid DNA amount. For the experiments a commercially available square-wave generator was applied. Post electroporation, the bovine fetal fibroblasts were observed after 24 h for viability and reporter expression. The best results were obtained with a single 10 millisecond square-wave pulse of 400 V using 10 μg supercoiled plasmid DNA and 0.3 × 10
conclusionsThe optimized electroporation conditions will be relevant for gene transfer experiments in bovine fetal fibroblasts to obtain genetically engineered donor cells for somatic cell nuclear transfer and for reprogramming experiments in this species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.