ArticleMolecules (Basel, Switzerland)2020
Hempseed Lignanamides Rich-Fraction: Chemical Investigation and Cytotoxicity towards U-87 Glioblastoma Cells.
Article in Molecules (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Metabolomic diversity and spatial localization of bioactive compounds in hemp achenes from diverse landraces.Journal of pharmaceutical analysis · 2026Article
- Comprehensive review on cannabis seed phenolic compounds from extraction to functional applications.Journal of cannabis research · 2026Review
- Optimizing ethanol-modified supercritical CO₂ extraction for enhanced bioactive compound recovery in hemp seed oil.Scientific reports · 2025Article
- Microwave-Assisted Extraction of Hemp Seed Oil: Process Optimization for Enhancing Oil Yield and Bioactive Compound Extractability.International journal of food science · 2025Article
- Current and Potential Use of Biologically Active Compounds Derived fromInternational journal of molecular sciences · 2024Review
- Polyphenol-rich fermented hempseed ethanol extracts improve obesity, oxidative stress, and neural health in high-glucose diet-inducedFood chemistry: X · 2024Article
- Exploring the neuroprotective activity of a lignanamides-rich extract in human neuroblastoma SH-SY5Y cells under dimethyl sulfoxide-induced stress.Frontiers in cell and developmental biology · 2024Article
- Hemp Seed Cake Flour as a Source of Proteins, Minerals and Polyphenols and Its Impact on the Nutritional, Sensorial and Technological Quality of Bread.Foods (Basel, Switzerland) · 2023Article
- Article
- Protein Mass Fingerprinting and Antioxidant Power of Hemp Seeds in Relation to Plant Cultivar and Environment.Plants (Basel, Switzerland) · 2023Article
- Mining the chemical diversity of the hemp seed (Frontiers in plant science · 2023Article
- Selected Seeds as Sources of Bioactive Compounds with Diverse Biological Activities.Nutrients · 2022Review
- Effect of Cannabidiolic Acid,Current issues in molecular biology · 2022Article
- Bioactive compounds from hemp (RSC advances · 2022Article
- Cytotoxic evaluation and chemical investigation of tomatoes from plants (Solanum lycopersicum L.) grown in uncontaminated and experimentally contaminated soils.Scientific reports · 2022Article
- Hemp Stem Epidermis and Cuticle: From Waste to Starter in Bio-Based Material Development.Polymers · 2022Article
- Identification ofMolecules (Basel, Switzerland) · 2022Article
- Cannabidiolic acid in Hemp Seed Oil Table Spoon and Beyond.Molecules (Basel, Switzerland) · 2022Article
- Characterization of Phenethyl Cinnamamide Compounds from Hemp Seed and Determination of Their Melanogenesis Inhibitory Activity.ACS omega · 2021Article
- Discovery of Active Ingredients Targeted TREM2 by SPR Biosensor-UPLC/MS Recognition System, and Investigating the Mechanism of Anti-Neuroinflammatory Activity on the Lignin-Amides fromMolecules (Basel, Switzerland) · 2021Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The weak but noteworthy presence of (poly)phenols in hemp seeds has been long overshadowed by the essential polyunsaturated fatty acids and digestible proteins, considered responsible for their high nutritional benefits. Instead, lignanamides and their biosynthetic precursors, phenylamides, seem to display interesting and diverse biological activities only partially clarified in the last decades. Herein, negative mode HR-MS/MS techniques were applied to the chemical investigation of a (poly)phenol-rich fraction, obtained from hemp seeds after extraction/fractionation steps. This extract contained phenylpropanoid amides and their random oxidative coupling derivatives, lignanamides, which were the most abundant compounds and showed a high chemical diversity, deeply unraveled through high resolution tandem mass spectrometry (HR-MS/MS) tools. The effect of different doses of the lignanamides-rich extract (LnHS) on U-87 glioblastoma cell line and non-tumorigenic human fibroblasts was evaluated. Thus, cell proliferation, genomic DNA damage, colony forming and wound repair capabilities were assessed, as well as LnHS outcome on the expression levels of pro-inflammatory cytokines. LnHS significantly inhibited U-87 cancer cell proliferation, but not that of fibroblasts, and was able to reduce U-87 cell migration, inducing further DNA damage. No modification in cytokines' expression level was found. Data acquired suggested that LnHS acted in U-87 cells by inducing the apoptosis machinery and suppressing the autophagic cell death.
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