ArticleJournal of thrombosis and haemostasis : JTH2020
A novel assay for studying the involvement of blood cells in whole blood thrombin generation.
Article in Journal of thrombosis and haemostasis : JTH, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Optical Biosensors for Blood Coagulation Monitoring: Advantages, Limitations, and Translational Potential.Biosensors · 2026Review
- Predicting dialyzer fiber blocking is hard due to high intrapatient variability and limited utility of thrombin generation markers.Scientific reports · 2026Article
- Cell-dependent antithrombotic effect of tranexamic acid.Frontiers in immunology · 2026Article
- Clinical and pathophysiological perspectives on haemostasis, inflammation, and thrombo-haemorrhagic risk in cirrhosis.EXCLI journal · 2026Review
- Pathogenic interplay between markedly elevated plasma lipoprotein(a) levels and prothrombotic mechanisms: a case report.Frontiers in cardiovascular medicine · 2026Article
- Feasibility to infer accurate fibrinogen concentrations from thrombin generation, prothrombin time, or Reptilase time waveforms.Research and practice in thrombosis and haemostasis · 2025Article
- Cryptogenic ischemic stroke in cardiac transthyretin amyloidosis and sinus rhythm: a case report.Frontiers in cardiovascular medicine · 2024Article
- Crucial roles of red blood cells and platelets in whole blood thrombin generation.Blood advances · 2023Article
- Venous Thromboembolism and Primary Thromboprophylaxis in Perioperative Pancreatic Cancer Care.Cancers · 2023Review
- C1 inhibitor deficiency enhances contact pathway-mediated activation of coagulation and venous thrombosis.Blood · 2023Article
- A novel mouse whole blood thrombin generation assay sensitive to FXI- and FIX-mediated amplification of coagulation.Blood advances · 2023Article
- Patients With Multiple Myeloma Have a Disbalanced Whole Blood Thrombin Generation Profile.Frontiers in cardiovascular medicine · 2022Article
- Assessing the individual roles of FII, FV, and FX activity in the thrombin generation process.Frontiers in cardiovascular medicine · 2022Article
- Intrinsic coagulation pathway-mediated thrombin generation in mouse whole blood.Frontiers in cardiovascular medicine · 2022Article
- Interplay between platelets and coagulation.Blood reviews · 2021Review
- Thrombocytopathies: Not Just Aggregation Defects-The Clinical Relevance of Procoagulant Platelets.Journal of clinical medicine · 2021Review
- A novel assay for studying the involvement of blood cells in whole blood thrombin generation.Journal of thrombosis and haemostasis : JTH · 2020Article
- Performance and reference intervals of thrombin generation test: results from the Brazilian longitudinal study of adult health (ELSA-Brasil). A cross-sectional study.Sao Paulo medical journal = Revista paulista de medicinaArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFluorogenic thrombin generation (TG) assays are commonly used to determine global coagulation phenotype in plasma. Whole blood (WB)-TG assays reach one step closer to physiology by involving the intrinsic blood cells, but erythrocytes cause variable quenching of the fluorescence signals, hampering its routine application.
objectiveTo develop a new assay for continuous WB-TG measurement.
methodsIn the new WB-TG assay, the erythrocyte-caused distortion of signal was solved by continuously mixing the sample during the measurement. The assay was validated by evaluating the reproducibility and comparing with the paper-based WB-TG assay. Reconstituted human blood and WB from 119 healthy donors was tested to explore the influences of hematocrit and platelet count on TG.
resultsThis novel WB-TG assay showed good reproducibility while being less affected by contact activation compared with the previous paper-based assay. Reconstitution experiments showed that the lag time of TG was shortened by the addition of platelets but not erythrocytes. Increasing hematocrit strongly augmented the peak thrombin, even in the presence of high platelet counts. The lag time and peak of WB-TG of 119 healthy donors were positively related to erythrocyte count after adjusting for age, sex, and oral contraceptive use with multiple linear regression analyses. The reference range and interindividual variation of WB-TG were determined in the healthy cohort.
conclusionsA novel WB-TG assay was developed, which is a straightforward tool to measure the involvement of platelets and erythrocytes in TG and may assist the research of blood cell-associated coagulation disorders.
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