ArticleJournal of leukocyte biology2020
Galectin-10, the protein that forms Charcot-Leyden crystals, is not stored in granules but resides in the peripheral cytoplasm of human eosinophils.
Article in Journal of leukocyte biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 57 citations in OpenAlex.
- Eosinophil biology in COPD: from scientific insight to clinical treatment action.ERJ open research · 2026Review
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- Mechanisms of Eosinophil Degranulation.Cells · 2026Review
- Glycobiology-driven therapeutic targeting of glycan-binding proteins: mechanisms, diseases, and clinical translation.Signal transduction and targeted therapy · 2026Review
- Crystalline Insights into Nasal Mucosa Inflammation and Remodeling: Unveiling Role of Galectin-10.Biomolecules · 2026Article
- Mechanisms, diagnosis, and treatment of olfactory dysfunction in rhinosinusitis.European journal of medical research · 2025Review
- Article
- Inhibition of EETosis with an anti-citrullinated histone antibody: a novel therapeutic approach for eosinophilic inflammatory disorders.Frontiers in immunology · 2025Article
- Eosinophil cytolysis with or without ETosis in four cases of human gastric cancer: a comparative ultrastructural study.Exploration of targeted anti-tumor therapy · 2025Article
- Galectin-10 Characterization in Cleft Lip Palate - Affected Palatal Tissue.Acta medica Lituanic · 2025Article
- Distribution of Immunomodulation, Protection and Regeneration Factors in Cleft-Affected Bone and Cartilage.Diagnostics (Basel, Switzerland) · 2024Article
- The epithelial era of asthma research: knowledge gaps and future direction for patient care.European respiratory review : an official journal of the European Respiratory Society · 2024Review
- Eosinophils as drivers of bacterial immunomodulation and persistence.Infection and immunity · 2024Review
- Eosinophil extracellular traps in respiratory ailment: Pathogenic mechanisms and clinical translation.World journal of otorhinolaryngology - head and neck surgery · 2024Review
- The Role of Galectins in Asthma Pathophysiology: A Comprehensive Review.Current issues in molecular biology · 2024Review
- An Emerging Role of Extracellular Traps in Chronic Rhinosinusitis.Current allergy and asthma reports · 2023Review
- Eosinophils in the tumor microenvironment: implications for cancer immunotherapy.Journal of translational medicine · 2023Review
- Extracellular distribution of galectin-10 in the esophageal mucosa of patients with eosinophilic esophagitis.Clinical and experimental immunology · 2023Article
- Glutathione disrupts galectin-10 Charcot-Leyden crystal formation to possibly ameliorate eosinophil-based diseases such as asthma.Acta biochimica et biophysica Sinica · 2023Article
- Lactose and Galactose Promote the Crystallization of Human Galectin-10.Molecules (Basel, Switzerland) · 2023Article
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
A predominant protein of human eosinophils is galectin-10 (Gal-10), also known as Charcot-Leyden crystal protein (CLC-P) because of its remarkable ability to form Charcot-Leyden crystals (CLCs), which are frequently found in tissues from patients with eosinophilic disorders. CLC-P/Gal-10 is highly expressed in human eosinophils and considered a biomarker of eosinophil involvement in inflammation. However, the intracellular sites where large pools of CLC-P/Gal-10 constitutively reside are still unclear, and whether this protein is derived or not from eosinophil granules remains to be established. Here, we applied pre-embedding immunonanogold transmission electron microscopy combined with strategies for optimal antigen and cell preservation and quantitative imaging analysis to investigate, for the first time, the intracellular localization of CLC-P/Gal-10 at high resolution in resting and activated human eosinophils. We demonstrated that CLC-P/Gal-10 is mostly stored in the peripheral cytoplasm of human eosinophils, being accumulated within an area of ∼250 nm wide underneath the plasma membrane and not within specific (secretory) granules, a pattern also observed by immunofluorescence. High-resolution analysis of single cells revealed that CLC-P/Gal-10 interacts with the plasma membrane with immunoreactive microdomains of high CLC-P/Gal-10 density being found in ∼60% of the membrane area. Eosinophil stimulation with CCL11 or TNF-α, which are known inducers of eosinophil secretion, did not change the peripheral localization of CLC-P/Gal-10 as observed by both immunofluorescence and immuno-EM (electron microscopy). Thus, in contrast to other preformed eosinophil proteins, CLC-P/Gal-10 neither is stored within secretory granules nor exported through classical degranulation mechanisms (piecemeal degranulation and compound exocytosis).
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