ArticleMicrobiologyOpen2020
Proteomic analysis of Aspergillus niger 3.316 under heat stress.
Article in MicrobiologyOpen, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Heat stress-induced degradation of glutamine synthetase rebalances central carbon-nitrogen metabolism and promotes thermotolerance inApplied and environmental microbiology · 2026Article
- Research progress on heat stress response mechanisms inFrontiers in microbiology · 2026Review
- Combined transcriptomics and metabolomics analyses reveal the molecular mechanism of heat tolerance inFrontiers in microbiology · 2025Article
- LC/MS Q-TOF Metabolomic Investigation of Amino Acids and Dipeptides inJournal of agricultural and food chemistry · 2022Article
- Integration of enzyme constraints in a genome-scale metabolic model of Aspergillus niger improves phenotype predictions.Microbial cell factories · 2021Article
- Article
- Novel Proteome and N-Glycoproteome of the Thermophilic FungusFrontiers in microbiology · 2021Article
- An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota.Life (Basel, Switzerland) · 2020Review
- Proteomic analysis of Aspergillus niger 3.316 under heat stress.MicrobiologyOpen · 2020Article
- Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
β-Glucosidase production by Aspergillus niger is accompanied by an inevitable temperature increase in the industrial fermentation environment. Hence, the synthetic process of β-glucosidase is negatively affected. However, our understanding of the heat stress response (HSR) mechanism in A. niger is still incomplete. The current study explored the intracellular proteome profile of A. niger 3.316 in group T (50°C stress) and group C (30°C control) using two proteomic approaches (isobaric tags for relative and absolute quantitation [iTRAQ] and label-free) and examined the expression of four proteins using a parallel reaction monitoring (PRM) approach. Based on the result of the iTRAQ proteomic analysis, 1,025 proteins were differentially expressed in group T compared to group C. Using the label-free approach, we only focused on 77 proteins with significant changes in their protein expression levels. In addition, we performed bioinformatics analysis on all these proteins and obtained detailed gene ontology (GO) enrichment and Kyoto encyclopedia of genes and genomes (KEGG) pathway results. Under heat stress conditions, the relative expression levels of proteins with protection and repair functions were upregulated in A. niger 3.316. These proteins were involved in metabolic pathways, oxidative phosphorylation, porphyrin and chlorophyll metabolism, pyruvate metabolism, and the citrate cycle (TCA cycle). The insights obtained from the presented proteomics and bioinformatics analyses can be used to further explore the HSR mechanism of A. niger.
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