Evidence map›Paper›PMID 32107645›Full record

ArticleWorld journal of microbiology & biotechnology2020

New engineered Geobacillus lipase GD-95RM for industry focusing on the cleaner production of fatty esters and household washing product formulations.

Gytis Druteika, Mikas Sadauskas, Vilius Malunavicius, Egle Lastauskiene, Roberta Statkeviciute, Agne Savickaite, Renata Gudiukaite

Abstract read
PubMed Publisher
In one paragraph

Article in World journal of microbiology & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Gytis DruteikaDepartment of Microbiology and Biotechnology, Institute of Biosciences, Life Sciences Center, Vilnius University, Sauletekis Avenue 7, Vilnius, 10257, Lithuania.
Mikas SadauskasInstitute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekis Avenue 7, Vilnius, 10257, Lithuania.
Vilius MalunaviciusDepartment of Microbiology and Biotechnology, Institute of Biosciences, Life Sciences Center, Vilnius University, Sauletekis Avenue 7, Vilnius, 10257, Lithuania.
Egle LastauskieneDepartment of Microbiology and Biotechnology, Institute of Biosciences, Life Sciences Center, Vilnius University, Sauletekis Avenue 7, Vilnius, 10257, Lithuania.
Roberta StatkeviciuteInstitute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekis Avenue 7, Vilnius, 10257, Lithuania.
Agne SavickaiteDepartment of Microbiology and Biotechnology, Institute of Biosciences, Life Sciences Center, Vilnius University, Sauletekis Avenue 7, Vilnius, 10257, Lithuania.
Renata GudiukaiteDepartment of Microbiology and Biotechnology, Institute of Biosciences, Life Sciences Center, Vilnius University, Sauletekis Avenue 7, Vilnius, 10257, Lithuania. renata.gudiukaite@gf.vu.lt.ORCID http://orcid.org/0000-0001-7839-727X
Vilnius University · LT

Funding

Lietuvos Mokslo Taryba 09.3.3.-LMT-K-712-09-0005
6 · The paper itself

Abstract

This study presents a new microbial lipolytic enzyme GD-95RM designed via random mutagenesis using previously characterized GD-95 lipase as a template. The improvement in activity of GD-95 lipase was caused by E100K, F154V and V174I mutations. Compared with GD-95 lipase, the GD-95RM lipase had 1.3-fold increased specific activity (2000 U/mg), demonstrated resistance to higher temperatures (75-85 °C), had fourfold increased V

Indexed as

MutationBacterial ProteinsButyratesEstersGeobacillusHot TemperatureHousehold ProductsLauratesLipaseModels, MolecularProtein EngineeringThermodynamicsBacterial ProteinsButyratesEstersLauratesLipaseBiotechnology of thermophilesCleaner ester synthesisGenotype–phenotype relationshipsGeobacillus lipasesProtein engineeringThermostable lipases

Identifiers

PMID32107645
OpenAlexW3007031676

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.