Evidence map›Paper›PMID 32107092›Full record

ArticleDiagnostic microbiology and infectious disease2020

Evaluation of a PCR-electrospray ionization mass spectrometry platform for detection and identification of fungal pathogens directly from prospectively collected bronchoalveolar lavage specimens.

Bei Jia, Robert Lovari, Heather Miller, David Metzgar, Christian Massire, Heather Carolan, Donna Toleno, Franco D'Alessio, Richard Rothman, Lawrence B Blyn and 1 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostic microbiology and infectious disease, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Bei JiaDivision of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Chongqing Key Laboratory of Infectious Diseases and Parasitic Diseases, Department of Infectious Diseases, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Robert LovariIbis Biosciences, Abbott, Carlsbad, CA, USA.
Heather MillerDivision of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
David MetzgarIbis Biosciences, Abbott, Carlsbad, CA, USA.
Christian MassireIbis Biosciences, Abbott, Carlsbad, CA, USA.
Heather CarolanIbis Biosciences, Abbott, Carlsbad, CA, USA.
Donna TolenoIbis Biosciences, Abbott, Carlsbad, CA, USA.
Franco D'AlessioDepartment pulmonary and critical care medicine.
Richard RothmanDepartment of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Lawrence B BlynIbis Biosciences, Abbott, Carlsbad, CA, USA.
Sean X ZhangDivision of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, MD, USA. Electronic address: szhang28@jhmi.edu.

Funding

NIAID NIH HHS HHSN272201400007C
6 · The paper itself

Abstract

The incidence of invasive fungal infections is on the rise worldwide due to the growth of the immunocompromised population. We report here the use of a diagnostic assay that utilizes a universal extraction method, broad spectrum PCR amplification and analysis via electrospray ionization mass spectrometry (PCR/ESI-MS) to detect and identify more than 200 pathogenic fungi directly from bronchoalveolar lavage (BAL) specimens in less than 8 hours. In this study, we describe both analytical and clinical performance of the assay, when run with prospectively collected clinical BAL specimens. In 146 patients with probable and possible fungal infections defined by EORTC/MSG (European Organization for Research and Treatment of Cancer/Mycoses Study Group) criteria, the PCR/ESI-MS assay demonstrated a sensitivity of 90.9% (95% CI: 76.4-96.9%) and a specificity of 82.3% (95% CI: 74.2-88.2%). This data demonstrates the utility of a non-culture based broad fungal targets molecular diagnostic tool for rapid and accurate diagnosis of invasive fungal infections in patients at risk of developing fungal diseases.

Indexed as

Polymerase Chain ReactionSpectrometry, Mass, Electrospray IonizationBronchoalveolar Lavage FluidFungiHumansInvasive Fungal InfectionsLimit of DetectionProspective StudiesSensitivity and SpecificityBronchoalveolar lavageFungal diagnosticFungal identificationFungal infectionsMass spectrometryPCR

Identifiers

PMID32107092
PMCPMC7127232

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.