Evidence map›Paper›PMID 32104410›Full record

ArticleAsian journal of pharmaceutical sciences2018

Intracellular distribution and internalization pathways of guanidinylated bioresponsive poly(amido amine)s in gene delivery.

Jinmin Zhang, Chunxi Wang, Mei Lu, Haonan Xing, Tianzhi Yang, Cuifang Cai, Xiaoyun Zhao, Minjie Wei, Jiankun Yu, Pingtian Ding

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Asian journal of pharmaceutical sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Jinmin ZhangSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Chunxi WangSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Mei LuSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Haonan XingSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Tianzhi YangDepartment of Basic Pharmaceutical Sciences, School of Pharmacy, Husson University, Bangor, ME, 04401, USA.
Cuifang CaiSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Xiaoyun ZhaoDepartment of Microbiology and Cell Biology, School of life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Minjie WeiDepartment of Pharmacology, School of Pharmacy, China Medical University, Shenyang, 110122, China.
Jiankun YuDepartment of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang, 110122, China.
Pingtian DingSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Shenyang Pharmaceutical University · CNChina Medical University · CNHusson University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Guanidinylated bioresponsive poly(amido amine)s polymers, CAR-CBA and CHL-CBA, were synthesized by Michael-type addition reaction between guanidine hydrochloride (CAR) or chlorhexidine (CHL) and N,N'-cystaminebisacrylamide (CBA). Previous studies have shown that both polymers had high transfection efficiencies as gene delivery carriers. In this study, we investigated the nucleolus localization abilities and cellular internalization pathways of these two polymers in gene delivery. Each polymer condensed plasmid DNA (pDNA) and formed nanoparticle complexes, and then their transfection studies were performed in MCF-7 cells. Both complexes were found enriched in nucleolus after cellular transfection, and their transfection efficiencies were significantly improved when transfection was performed on MCF-7 cells arrested at M phase. The transfection efficiency of CAR-CBA-pDNA was inhibited by chlorpromazine, and cell endosomes were disrupted after being exposed to CAR-CBA-pDNA. In regards to CHL-CBA-pDNA, its transfection efficiency was not affected by three types of endocytosis inhibitors used in the study, and CHL-CBA-pDNA showed no effect on endosomes. Cellular lactate dehydrogenase release and membrane morphology were changed after cells were transfected by the two complexes. The results indicated that both CAR-CBA and CHL-CBA polymers demonstrated good nucleolus localization abilities. It was beneficial for transfection when cells were arrested at M phase. CAR-CBA-pDNA cellular internalization was involved with clathrin-mediated endocytosis pathway, and escaping from endosomal entrapment, while the cellular uptake of CHL-CBA-pDNA occurs via clathrin- and caveolae-independent mechanism.

Indexed as

AFM, atomic force microscopyCAR, guanidine hydrochlorideCBA, N,N’-cystaminebisacrylamideCell cycle statusCHL, chlorhexidineCPPs, cell- penetrating peptidesDAPI, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochlorideDiI, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorateDLS, dynamic light scatteringDMEM, Dulbecco's Modification of Eagle's mediumEGFP, enhanced green fluorescent proteinGuanidinylated poly(amido amine)s polymersGua-SS-PAAs, guanidinylated disulfide containing poly(amido amine) polymersInternalization pathwaysLDH, Lactate dehydrogenaseNMR, nuclear magnetic resonanceNOR, nucleolar organizing regionNucleolus localizationOD, optical densityPAAs, poly(amido, amine)spDNA, plasmid DNArRNA, ribosomal RNASS-PAAs, disulfide containing poly(amido, amine)

Identifiers

PMID32104410
PMCPMC7032094
OpenAlexW2789924254

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.