ArticleBMC research notes2020
Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies.
Article in BMC research notes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Establishment ofPlants (Basel, Switzerland) · 2025Article
- Combining biotechnology with sustainability: feasibility of bioenergy sorghum in generating high-value bioproducts.Biotechnology letters · 2025Review
- Targeted seed EMS mutagenesis reveals a basic helix-loop-helix transcription factor underlying male sterility in sorghum.Genetics · 2025Article
- Through the lens of bioenergy crops: advances, bottlenecks, and promises of plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- Plant engineering: advances, bottlenecks, and promise.The Plant journal : for cell and molecular biology · 2025Article
- Development and optimization of in vitro shoot regeneration in Physalis peruviana using cotyledon explants.Journal, genetic engineering & biotechnology · 2025Article
- Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.The Plant journal : for cell and molecular biology · 2025Article
- Plant Engineering to Enable Platforms for Sustainable Bioproduction of Terpenoids.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Isolation, Purification, and Application of Protoplasts and Transient Expression Systems in Plants.International journal of molecular sciences · 2023Review
- Agrobacterium-mediated in planta transformation of cut coleoptile: a new, simplified, and tissue culture-independent method to deliver the CRISPR/Cas9 system in rice.Molecular biology reports · 2023Article
- Identification of a suitable method of inoculation for reducing background effect in mock-inoculated controls during gene expression studies inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- An efficient method for protoplast-mediated production of transformed castor bean (Ricinus communis) lines.BMC research notes · 2023Article
- New methods for sorghum transformation in temperate climates.AoB PLANTS · 2023Review
- Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy.Journal of experimental botany · 2022Review
- Development and Adoption of Genetically Engineered Plants for Virus Resistance: Advances, Opportunities and Challenges.Plants (Basel, Switzerland) · 2021Review
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- Leaf infiltration in plant science: old method, new possibilities.Plant methods · 2021Review
- Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement.Frontiers in plant science · 2021Review
- A Rapid and Highly Efficient Method for Transient Gene Expression in Rice Plants.Frontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
objectivesSorghum is one of the most recalcitrant species for transformation. Considering the time and effort required for stable transformation in sorghum, establishing a transient system to screen the efficiency and full functionality of vector constructs is highly desirable.
resultsHere, we report an Agrobacterium-mediated transient transformation assay with intact sorghum leaves using green fluorescent protein as marker. It also provides a good monocot alternative to tobacco and protoplast assays with a direct, native and more reliable system for testing single guide RNA (sgRNA) expression construct efficiency. Given the simplicity and ease of transformation, high reproducibility, and ability to test large constructs, this method can be widely adopted to speed up functional genomic and genome editing studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.