Evidence map›Paper›PMID 32103775›Full record

ArticleBMC research notes2020

Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens.

Bahman Mirzaei, Peyman Faridifar, Mostafa Shahmoradi, Reza Shapouri, Fatemeh Iranpour, Fakhri Haghi, Mahnaz Ezzedin, Reyhaneh Babaei, Seyed Fazlollah Mousavi

Open access · goldAbstract read
In one paragraph

Article in BMC research notes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Bahman MirzaeiDepartment of Microbiology and Virology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran.
Peyman FaridifarDepartment of Microbiology, Islamic Azad University, Zanjan Branch, Zanjan, Iran.
Mostafa ShahmoradiDepartment of Microbiology, Islamic Azad University, Zanjan Branch, Zanjan, Iran.
Reza ShapouriDepartment of Microbiology, Islamic Azad University, Zanjan Branch, Zanjan, Iran.
Fatemeh IranpourDepartment of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Fakhri HaghiDepartment of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Mahnaz EzzedinDepartment of Microbiology, Islamic Azad University, Tehran Branch, Tehran, Iran.
Reyhaneh BabaeiDepartment of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Seyed Fazlollah MousaviDepartment of Microbiology, Microbial Research Center, Pasteur Institute of Iran, Tehran, Iran. Mousavi@pasteur.ac.ir.
Zanjan University of Medical Sciences · IRIslamic Azad University of Zanjan · IRPasteur Institute of Iran · IRIslamic Azad University, Tehran · IRMazandaran University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesStaphylococcus epidermidis is the primary causative agent of infections associated with indwelling biomaterials. Antibiotic susceptibility patterns, Biofilm formation capability, and screening of responsible genes in biofilm formation procedure in clinical isolates (icaA, icaB, icaC, icaD, sdrG, and atlE) were assigned as the main objectives in this study. The clinical samples were analyzed via standard biochemical assays for identifying different bacteria which were confirmed using the multiplex colony PCR method. Subsequently, biofilm-formation capability, antibiotic susceptibility testing, and the frequency of genes responsible for biofilm formation in the confirmed strains were checked.

resultsOut of 183 clinical specimens 54 S. epidermidis isolates were detected by targeting a housekeeping gene (sesc) taking advantage of the PCR procedure. All of the strains were Biofilm forming producers. The in vitro biofilm formation assays determined that 45 (83.33%), 5 (9.26%), 4 (7.41%) were strong, moderate, and weak biofilm former strains respectively. Among the isolated strains, the specific frequencies of the biofilm-forming genes were specified to be (98%) for sdrG, (84%) for atlE, (80%) for icaC, and (70%) for icaD. Cefamandole and Amikacin are the most effective antibiotics in isolated strains. All strains were ascertained to be methicillin and amoxicillin/clavulanic acid resistant.

Indexed as

BiofilmsAnti-Bacterial AgentsBacterial ProteinsDrug Resistance, BacterialGenotypeHumansMicrobial Sensitivity TestsPhenotypePolymerase Chain ReactionProsthesis-Related InfectionsStaphylococcal InfectionsStaphylococcus epidermidisAnti-Bacterial AgentsBacterial ProteinsatlEBiofilm-related genesicaADCBMultiplex colony PCRsdrGStaphylococcus epidermidis

Identifiers

PMID32103775
PMCPMC7045379
OpenAlexW3012681344

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.