ArticleMicrobial cell factories2020
A modular pathway engineering strategy for the high-level production of β-ionone in Yarrowia lipolytica.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 93 citations in OpenAlex.
- Research progress on metabolic engineering for efficient squalene synthesis in yeast cell factories.Biotechnology letters · 2026Review
- Engineered microorganisms unlock a new era of diterpenoid biomanufacturing: recent advances and future perspectives.Bioresources and bioprocessing · 2026Review
- Engineering Modification for the Efficient Synthesis of β-ionone by Yarrowia lipolytica.Biotechnology journal · 2026Article
- Efficient biosynthesis of the plant-derived diterpenoid 14-hydroxy-dehydroabietadiene in Saccharomyces cerevisiae.Microbial cell factories · 2026Article
- Advances in the sustainable biosynthesis of valuable terpenoid flavor compounds and precursors in micro-organisms.Biotechnology for biofuels and bioproducts · 2025Review
- Identification, Cloning, and Functional Characterization of Carotenoid Cleavage Dioxygenase (CCD) fromBiology · 2025Article
- Enhancing cellulase biosynthesis ofiScience · 2025Article
- Introduction of human mMicrobial cell factories · 2025Article
- Beyond pigments and perfumes: engineering in the carotenoid and apocarotenoid spectrum, novel enzymes, and synthetic biology strategies.Frontiers in bioengineering and biotechnology · 2025Review
- Synthesis of β-ionone from xylose and lignocellulosic hydrolysate in genetically engineered oleaginous yeast Yarrowia lipolytica.Biotechnology letters · 2024Article
- New insights into the roles of fungi and bacteria in the development of medicinal plant.Journal of advanced research · 2024Review
- Engineering a non-model yeastSynthetic and systems biotechnology · 2024Article
- Diverse roles played by "Pseudomonas fluorescens complex" volatile compounds in their interaction with phytopathogenic microrganims, pests and plants.World journal of microbiology & biotechnology · 2024Review
- Progress in the Metabolic Engineering ofBiodesign research · 2024Review
- Multidimensional Optimization ofBiodesign research · 2024Article
- Refactoring the architecture of a polyketide gene cluster enhances docosahexaenoic acid production in Yarrowia lipolytica through improved expression and genetic stability.Microbial cell factories · 2023Article
- Engineering ofMetabolic engineering communications · 2022Review
- Characteristics of a new carotenoid cleavage dioxygenase NtCCD10 derived from Nicotiana tabacum.Planta · 2022Article
- Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories.Bioresources and bioprocessing · 2022Review
- Efficient production of the β-ionone aroma compound from organic waste hydrolysates using an engineeredFrontiers in microbiology · 2022Article
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4 authors at 1 institution in 1 country.
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Abstract
backgroundThe GRAS and oleaginous yeast Yarrowia lipolytica (Y. lipolytica) is an attractive cell factory for the production of chemicals and biofuels. The production of many natural products of commercial interest have been investigated in this cell factory by introducing heterologous biosynthetic pathways and by modifying the endogenous pathways. However, since natural products anabolism involves long pathways and complex regulation, re-channelling carbon into the product of target compounds is still a cumbersome work, and often resulting in low production performance.
resultsIn this work, the carotenogenic genes contained carB and bi-functional carRP from Mucor circinelloides and carotenoid cleavage dioxygenase 1 (CCD1) from Petunia hybrida were introduced to Y. lipolytica and led to the low production of β-ionone of 3.5 mg/L. To further improve the β-ionone synthesis, we implemented a modular engineering strategy for the construction and optimization of a biosynthetic pathway for the overproduction of β-ionone in Y. lipolytica. The strategy involved the enhancement of the cytosolic acetyl-CoA supply and the increase of MVA pathway flux, yielding a β-ionone titer of 358 mg/L in shake-flask fermentation and approximately 1 g/L (~ 280-fold higher than the baseline strain) in fed-batch fermentation.
conclusionsAn efficient β-ionone producing GRAS Y. lipolytica platform was constructed by combining integrated overexpressed of heterologous and native genes. A modular engineering strategy involved the optimization pathway and fermentation condition was investigated in the engineered strain and the highest β-ionone titer reported to date by a cell factory was achieved. This effective strategy can be adapted to enhance the biosynthesis of other terpenoids in Y. lipolytica.
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