Evidence map›Paper›PMID 32103460›Full record

ArticleJournal of industrial microbiology & biotechnology2020

Investigation of secondary metabolism in the industrial butanol hyper-producer Clostridium saccharoperbutylacetonicum N1-4.

Jeffrey S Li, Colin C Barber, Nicolaus A Herman, Wenlong Cai, Ella Zafrir, Yongle Du, Xuejun Zhu, Will Skyrud, Wenjun Zhang

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Sporulation in solventogenic and acetogenic clostridia.Applied microbiology and biotechnology · 2021
    Review
  6. Article
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Jeffrey S LiDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
Colin C BarberDepartment of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA, USA.
Nicolaus A HermanDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
Wenlong CaiDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
Ella ZafrirDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
Yongle DuDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
Xuejun ZhuDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
Will SkyrudDepartment of Chemistry, University of California, Berkeley, CA, USA.
Wenjun ZhangDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA. wjzhang@berkeley.edu.ORCID http://orcid.org/0000-0001-9348-972X
University of California, Berkeley · US

Funding

In Situ Natural Product Labeling and ApplicationsDP2AT009148 · NCCIH · UNIVERSITY OF CALIFORNIA BERKELEY · PI ZHANG, WENJUN · 2015 to 2020
$2.8M
NCCIH NIH HHS DP2 AT009148NIH HHS DP2AT009148
6 · The paper itself

Abstract

Clostridium saccharoperbutylacetonicum N1-4 (Csa) is a historically significant anaerobic bacterium which can perform saccharolytic fermentations to produce acetone, butanol, and ethanol (ABE). Recent genomic analyses have highlighted this organism's potential to produce polyketide and nonribosomal peptide secondary metabolites, but little is known regarding the identity and function of these metabolites. This study provides a detailed bioinformatic analysis of seven biosynthetic gene clusters (BGCs) present in the Csa genome that are predicted to produce polyketides/nonribosomal peptides. An RNA-seq-based untargeted transcriptomic approach revealed that five of seven BGCs were expressed during ABE fermentation. Additional characterization of a highly expressed nonribosomal peptide synthetase gene led to the discovery of its associated metabolite and its biosynthetic pathway. Transcriptomic analysis suggested an association of this nonribosomal peptide synthetase gene with butanol tolerance, which was supported by butanol challenge assays.

Indexed as

Secondary MetabolismAcetoneButanolsClostridiumEthanolFermentationAcetoneButanolsEthanolABE fermentationAnaerobeNonribosomal peptide synthetasePolyketide synthaseTranscriptomics

Identifiers

PMID32103460
PMCPMC7665961
OpenAlexW3007946133

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.