ArticleNucleic acids research2020
Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed.
- Point of Care Testing and Recombinase Polymerase Amplification.Journal of clinical laboratory analysis · 2026Article
- Molecular Diagnostics of Infectious Diseases.Yonago acta medica · 2026Review
- ViraLite: an ultracompact HIV viral load self-testing system with internal quality control.Microsystems & nanoengineering · 2026Article
- A Novel Voltammetric Point-of-Care Device for Rapid and Accurate Nucleic Acid and Viral Pathogen Detection.ACS omega · 2026Article
- Article
- Elimination of cis-cleavage in CRISPR diagnostics for one-pot rapid nucleic acid detection.Nucleic acids research · 2026Article
- Digital CRISPR-based diagnostics for quantification of Candida auris and resistance mutations.Nature biomedical engineering · 2026Article
- A universal kinetic framework for quantitative isothermal amplification governed by polymerase speed, amplicon size, and binding efficiency.Nucleic acids research · 2026Article
- Recent advances in isothermal amplification techniques for detection of animal diseases.Frontiers in veterinary science · 2026Review
- Generation of high-quality single-stranded DNA for full-length and truncated genome standards of recombinant adeno-associated viruses.Molecular therapy. Methods & clinical development · 2025Article
- Asymmetric volume-mediated buffer control overcomes sensitivity limits in one-pot RAA-CRISPR/Cas12a visual detection.Analytical and bioanalytical chemistry · 2025Article
- Machine learning assisted CRCA multi-color fluorescent droplet imaging for high-sensitivity simultaneous detection of Maillard hazards.iScience · 2025Article
- Rapid Visual Detection of Senecavirus A Based on RPA-CRISPR/Cas12a System with Canonical or Suboptimal PAM.Viruses · 2025Article
- ViraLite: An Ultracompact HIV Viral Load Self-Testing System with Internal Quality Control.medRxiv : the preprint server for health sciences · 2025Article
- A duplex real-time loop-mediated isothermal amplification assay for simultaneous detection of elephant endotheliotropic herpesvirus types 1 and 5.Frontiers in veterinary science · 2025Article
- A TtAgo-mediated LAMP System for highly sensitive and specific detection of rotavirus in pediatric diarrheal samples.Frontiers in cellular and infection microbiology · 2025Article
- The dark side of avocados: a review of anthracnose and stem-end rot in postharvest fruit.Frontiers in microbiology · 2025Review
- Validation of a Novel Strategy for Fluorescence Quenching for a Self-Quenching Fluorogenic Probe and Its Application for Visual Loop-Mediated Isothermal Amplification Detection During Food Safety Analysis.Foods (Basel, Switzerland) · 2024Article
- Mimicking a Cellular Crowding Environment for Enzyme-Free Paper-Based Nucleic Acid Tests at the Point of Care.ACS sensors · 2024Article
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
10 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
Abstract
Isothermal amplification assays, such as loop-mediated isothermal amplification (LAMP), show great utility for the development of rapid diagnostics for infectious diseases because they have high sensitivity, pathogen-specificity and potential for implementation at the point of care. However, elimination of non-specific amplification remains a key challenge for the optimization of LAMP assays. Here, using chlamydia DNA as a clinically relevant target and high-throughput sequencing as an analytical tool, we investigate a potential mechanism of non-specific amplification. We then develop a real-time digital LAMP (dLAMP) with high-resolution melting temperature (HRM) analysis and use this single-molecule approach to analyze approximately 1.2 million amplification events. We show that single-molecule HRM provides insight into specific and non-specific amplification in LAMP that are difficult to deduce from bulk measurements. We use real-time dLAMP with HRM to evaluate differences between polymerase enzymes, the impact of assay parameters (e.g. time, rate or florescence intensity), and the effect background human DNA. By differentiating true and false positives, HRM enables determination of the optimal assay and analysis parameters that leads to the lowest limit of detection (LOD) in a digital isothermal amplification assay.
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