ArticleNature communications2020
Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- Water-Derived Radical Species Initiate Six-Electron Oxidation of Cyclic Ketones in Microdroplets.Angewandte Chemie (International ed. in English) · 2026Article
- Enhancing Enzyme Activity Via Noncovalent Association with DNA-Functionalized Nanoparticles.JACS Au · 2026Article
- Accelerated Chemistry in Microdroplets.Accounts of chemical research · 2026Article
- A Unified Mass Spectrometry Workflow Integrating Bottom-Up and Native Top-Down Proteomics via Nanodroplet Enzymatic Digestion.Analytical chemistry · 2026Article
- Ultrafast Postcolumn Microdroplet Derivatization of Nucleobases for Enhanced Online Detection, Characterization, and Quantification of Nucleic Acid Modifications Using LC-MSAnalytical chemistry · 2026Article
- Dark Reactions in Microdroplets Explain Widespread Artifacts in Metabolomic Profiling.ACS measurement science au · 2026Article
- Enhancing Proteoform Sequence Coverage Using Top-Down Mass Spectrometry with In-Source Fragmentation and Middle-Down Mass Spectrometry.Analytical chemistry · 2026Article
- Accelerated and Green Synthesis ofJournal of the American Chemical Society · 2026Article
- Chemoselective cyclodesulfurizationChemical science · 2025Article
- Mechanisms of ionization and of chemical reactions in charged microdroplets.Chemical science · 2025Review
- Ultrafast Microdroplet Digestion of Antibodies with Fc-Silencing Mutations.Analytical chemistry · 2025Article
- Interfacial electromigration for accelerated reactions.Analytica chimica acta · 2025Article
- Deciphering the mechanism of hydrogen peroxide formation in ultrasound-mediated water-in-oil microdroplets.Chemical science · 2025Article
- Increasing Proteome Coverage Through a Reduction in Analyte Complexity in Single-Cell Equivalent Samples.Journal of proteome research · 2025Article
- Rapid and Sensitive Chemical Analysis of Individual Picolitre Droplets by Mass Spectrometry.Analytical chemistry · 2025Article
- Challenges and Insights in Absolute Quantification of Recombinant Therapeutic Antibodies by Mass Spectrometry: An Introductory Review.Antibodies (Basel, Switzerland) · 2025Review
- Unlocking the electrochemical functions of biomolecular condensates.Nature chemical biology · 2024Review
- Reaction acceleration at the surface of a levitated droplet by vapor dosing from a partner droplet.Chemical science · 2024Article
- Dielectrophoretic bead-droplet reactor for solid-phase synthesis.Nature communications · 2024Article
- Accelerating protein aggregation and amyloid fibrillation for rapid inhibitor screening.Chemical science · 2024Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymatic digestion for protein sequencing usually requires much time, and does not always result in high sequence coverage. Here we report the use of aqueous microdroplets to accelerate enzymatic reactions and, in particular, to improve protein sequencing. When a room temperature aqueous solution containing 10 µM myoglobin and 5 µg mL
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.