Evidence map›Paper›PMID 32102854›Full record

ArticleJournal of clinical microbiology2020

Recombinase Polymerase Amplification and Lateral Flow Assay for Ultrasensitive Detection of Low-Density Plasmodium falciparum Infection from Controlled Human Malaria Infection Studies and Naturally Acquired Infections.

Albert Lalremruata, The Trong Nguyen, Matthew B B McCall, Ghyslain Mombo-Ngoma, Selidji T Agnandji, Ayôla A Adegnika, Bertrand Lell, Michael Ramharter, Stephen L Hoffman, Peter G Kremsner and 1 more

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

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  13. Plant pest surveillance: from satellites to molecules.Emerging topics in life sciences · 2021
    Review
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  16. Article
  17. Preliminary Evaluation of Rapid Visual Identification ofFrontiers in cellular and infection microbiology · 2021
    Article
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Albert LalremruataInstitut für Tropenmedizin, Universität Tübingen and German Center for Infection Research, Tübingen, Germany.
The Trong NguyenInstitut für Tropenmedizin, Universität Tübingen and German Center for Infection Research, Tübingen, Germany.
Matthew B B McCallInstitut für Tropenmedizin, Universität Tübingen and German Center for Infection Research, Tübingen, Germany.
Ghyslain Mombo-NgomaInstitut für Tropenmedizin, Universität Tübingen and German Center for Infection Research, Tübingen, Germany.
Selidji T AgnandjiInstitut für Tropenmedizin, Universität Tübingen and German Center for Infection Research, Tübingen, Germany.
Ayôla A AdegnikaInstitut für Tropenmedizin, Universität Tübingen and German Center for Infection Research, Tübingen, Germany.
Bertrand LellInstitut für Tropenmedizin, Universität Tübingen and German Center for Infection Research, Tübingen, Germany.
Michael RamharterCentre de Recherches Médicales de Lambaréné and African Partner Institution, German Center for Infection Research, Lambaréné, Gabon.
Stephen L HoffmanSanaria Inc., Rockville, Maryland, USA.
Peter G KremsnerInstitut für Tropenmedizin, Universität Tübingen and German Center for Infection Research, Tübingen, Germany.
Benjamin MordmüllerInstitut für Tropenmedizin, Universität Tübingen and German Center for Infection Research, Tübingen, Germany benjamin.mordmueller@uni-tuebingen.de.

Funding

Plasmodium Falciparum Whole Sporozoite Malaria VaccineR44AI055229 · NIAID · SANARIA, INC. · PI HOFFMAN, STEPHEN LEV · 2005 to 2015
$10.0M
Universal Attenuated Sporozoite Vaccine and Challenge SystemR44AI058375 · NIAID · SANARIA, INC. · PI HOFFMAN, STEPHEN LEV · 2006 to 2018
$9.9M
NIAID NIH HHS R44 AI055229NIAID NIH HHS R44 AI058375
6 · The paper itself

Abstract

Microscopy and rapid diagnostic tests (RDTs) are the main diagnostic tools for malaria but fail to detect low-density parasitemias that are important for maintaining malaria transmission. To complement existing diagnostic methods, an isothermal reverse transcription-recombinase polymerase amplification and lateral flow assay (RT-RPA) was developed. We compared the performance with that of ultrasensitive reverse transcription-quantitative PCR (uRT-qPCR) using nucleic acid extracts from blood samples (

Indexed as

MalariaMalaria, FalciparumGabonHumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPlasmodium falciparumRecombinasesSensitivity and SpecificityRecombinasesCHMIdiagnosticseliminationisothermalmass screen and treatPlasmodium falciparumsubmicroscopic

Identifiers

PMID32102854
PMCPMC7180247

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.