SynthesisStem cell research & therapy2020
The necessity for standardization of glioma stem cell culture: a systematic review.
Synthesis in Stem cell research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Article
- Patient-derived spheroid culture: A promising in vitro model for drug screening in retinoblastoma.Medical oncology (Northwood, London, England) · 2026Article
- Primary glioma cell culture: from monolayer to organoids.Protoplasma · 2026Review
- Canine organoids: state-of-the-art, translation potential for human medicine and plea for standardization.Frontiers in veterinary science · 2025Review
- Understanding current experimental models of glioblastoma-brain microenvironment interactions.Journal of neuro-oncology · 2024Review
- Enteric neurospheres retain the capacity to assemble neural networks with motile and metamorphic gliocytes and ganglia.Stem cell research & therapy · 2023Article
- A systematic study on the use of multifunctional nanodiamonds for neuritogenesis and super-resolution imaging.Biomaterials research · 2023Article
- Isolation of Cells from Glioblastoma Multiforme Grade 4 Tumors for Infection with Zika Virus prME and ME Pseudotyped HIV-1.International journal of molecular sciences · 2023Article
- Transcriptome Changes in Glioma Cells Cultivated under Conditions of Neurosphere Formation.Cells · 2022Article
- Tailoring drug co-delivery nanosystem for mitigating U-87 stem cells drug resistance.Drug delivery and translational research · 2022Article
- Article
- P4HA1 Regulates CD31Frontiers in oncology · 2022Article
- Short- and long-term efficacy of sustained-release chemotherapy in tumor bed interstitium combined with surgical resection for recurrent malignant glioma.American journal of translational research · 2022Article
- Dual role of WNT5A in promoting endothelial differentiation of glioma stem cells and angiogenesis of glioma derived endothelial cells.Oncogene · 2021Article
- GASC1 promotes glioma progression by enhancing NOTCH1 signaling.Molecular medicine reports · 2021Article
- Pre-clinical tumor models of primary brain tumors: Challenges and opportunities.Biochimica et biophysica acta. Reviews on cancer · 2021Review
- A Fast and Efficient Approach to Obtaining High-Purity Glioma Stem Cell Culture.Frontiers in genetics · 2021Article
- A machine learning-assisted systematic review of preclinical glioma modeling: Is practice changing with the times?Neuro-oncology advancesArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe cancer stem cell hypothesis is an old idea which has been revived in recent years for many cancers, including gliomas. However, this concept has become controversial due to a series of studies with conflicting results.
methodsA systematic literature search was conducted in PubMed and the Web of Science database to analyze studies using serum-free medium and its components in glioma stem cells, glioma stem-like cells, glioma-initiating cells, or glioma neurosphere cultures. All the studies reviewed were published between 1970 and 2019. We found that no standardized culture method was used, and the data were incomparable due to differing culture conditions and the use of media with different components.
conclusionsHere, we review the most commonly used serum-free media and added components for glioma stem cell culture while highlighting the function of each component used in the media. We emphasize the necessity for standardization of glioma stem cell culture and propose a standard culture medium to prevent bias in glioma stem cell research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.