Evidence map›Paper›PMID 32102514›Full record

ArticleAsian Pacific journal of cancer prevention : APJCP2020

Cyclin D1 Gene Numerical Imbalances in Laryngeal Squamous Cell Carcinoma: A Tissue Microarray Grid Based Analysis.

Efthymios Kyrodimos, Vasileios Papanikolaou, Evangelos Tsiambas, Dimitrios Kikidis, Dimitrios Peschos, Vasileios Ragos, Nicholas Mastronikolis, Christos Riziotis, Aristeidis Chrysovergis

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Article in Asian Pacific journal of cancer prevention : APJCP, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. DIAPH2 gene polymorphisms and laryngeal cancer risk in men.Journal of cancer research and clinical oncology · 2024
    Article
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Efthymios Kyrodimos1st ENT Dept, Hippocration Hospital, Medical School, University of Athens, Athens, Greece.
Vasileios Papanikolaou1st ENT Dept, Hippocration Hospital, Medical School, University of Athens, Athens, Greece.
Evangelos TsiambasDepartment of Pathology-Cytology, 401 GAH, Athens, Greece.
Dimitrios Kikidis1st ENT Dept, Hippocration Hospital, Medical School, University of Athens, Athens, Greece.
Dimitrios PeschosDepartment of Physiology, Medical School, University of Ioannina, Greece.
Vasileios RagosDepartment of Maxillofacial, Medical School, University of Ioannina, Greece.
Nicholas MastronikolisDepartment of Otorhinolaryngology, Head and Neck Surgery, Medical School, University of Patras, Greece.
Christos RiziotisTheoretical and Physical Chemistry Institute, Photonics for Nanoapplications Laboratory, National Hellenic Research Foundation, Athens, Greece.
Aristeidis Chrysovergis1st ENT Dept, Hippocration Hospital, Medical School, University of Athens, Athens, Greece.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDeregulation of critical proteins involved in cell cycle stability, such as cyclins, is a frequent genetic event in the development and progression of solid malignancies. Concerning laryngeal squamous cell carcinoma (LSCC), cyclin D1 oncogenic transformation leads to an aberrant protein expression and seems to affect the biological behaviour of the neoplasm. The aim of this study was to determine the correlation of cyclin D1 numerical imbalances with the corresponding protein expression levels in LSCCs. MATERIAL AND

methodUsing tissue microarray (TMA) technology, fifty (n=50) histologically confirmed primary LSSCs were cored at a diameter of 1.5 mm. Immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) analyses were applied. Concerning the screening process in CISH slides, a novel real-time reference and calibration grid platform was implemented.

resultsProtein overexpression was observed in 22/50 (44%) cases; whereas, gene amplification was seen in 13/50 (26%) cases (p=0.02). Combined protein/ gene deregulation was associated with the stage of malignancy (p= 0.0014, p=0.001), whereas overall protein expression was strongly correlated with the grade of tumour (p= 0.001).

conclusionCyclin D1 gene amplification led to aberrant protein expression in LSCCs and it was also correlated with an aggressive biological behaviour. To best of our knowledge, this study was the first described grid based CISH analysis under conventional bright field microscopy for detecting gene numerical imbalances while providing a novel and accurate description for screening-mapping process in the entire slide area.<br />.

Indexed as

Gene AmplificationAgedBiomarkers, TumorCarcinoma, Squamous CellCyclin D1FemaleFollow-Up StudiesHumansLaryngeal NeoplasmsMaleMiddle AgedPrognosisTissue Array AnalysisBiomarkers, TumorCCND1 protein, humanCyclin D1CarcinomacyclinGenegridLarynx

Identifiers

PMID32102514
PMCPMC7332136

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.