Evidence map›Paper›PMID 32101717›Full record

ArticleBiophysical journal2020

Facile Characterization of Topology of DNA Catenanes.

Lin Li, Ran An, Jiaxuan Tang, Zhe Sui, Guoqing Wang, Makoto Komiyama, Xingguo Liang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Lin LiCollege of Food Science and Engineering, Ocean University of China, Nucleic acids Chemistry and Biotechnology Laboratory, Shinan-qu, Qingdao, China.
Ran AnCollege of Food Science and Engineering, Ocean University of China, Nucleic acids Chemistry and Biotechnology Laboratory, Shinan-qu, Qingdao, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Jiaxuan TangCollege of Food Science and Engineering, Ocean University of China, Nucleic acids Chemistry and Biotechnology Laboratory, Shinan-qu, Qingdao, China.
Zhe SuiCollege of Food Science and Engineering, Ocean University of China, Nucleic acids Chemistry and Biotechnology Laboratory, Shinan-qu, Qingdao, China.
Guoqing WangCollege of Food Science and Engineering, Ocean University of China, Nucleic acids Chemistry and Biotechnology Laboratory, Shinan-qu, Qingdao, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Makoto KomiyamaCollege of Food Science and Engineering, Ocean University of China, Nucleic acids Chemistry and Biotechnology Laboratory, Shinan-qu, Qingdao, China. Electronic address: xgsz@ouc.edu.cn.
Xingguo LiangCollege of Food Science and Engineering, Ocean University of China, Nucleic acids Chemistry and Biotechnology Laboratory, Shinan-qu, Qingdao, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address: liangxg@ouc.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During the preparation of single-stranded DNA catenanes, topological isomers of different linking numbers (Lk) are intrinsically produced, and they must be separated from each other to construct sophisticated nanostructures accurately. In many previous studies, however, mixtures of these isomers were directly employed to construct nanostructures without sufficient characterization. Here, we present a method that easily and clearly characterizes the isomers by polyacrylamide gel electrophoresis. To the mixtures of topological isomers of [2]catenanes, two-strut oligonucleotides, which are complementary with a part of both rings, were added to connect the rings and fix the whole conformations of isomers. As a result, the order of migration rate was always Lk3 > Lk2 > Lk1, irrespective of gel concentration. Thus, all the topological isomers were unanimously characterized by only one polyacrylamide gel electrophoresis experiment. Well-characterized DNA catenanes are obtainable by this two-strut strategy, opening the way to more advanced nanotechnology.

Indexed as

DNA, CatenatedNanostructuresDNA, Single-StrandedNanotechnologyNucleic Acid ConformationOligonucleotidesDNA, CatenatedDNA, Single-StrandedOligonucleotides

Identifiers

PMID32101717
PMCPMC7136276

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