ArticleAMB Express2020
Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris.
Article in AMB Express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 73 citations in OpenAlex.
- Thinking big in small scale: characterization of peroxidase production in Komagataella phaffii using two-compartment scale-down system.Bioprocess and biosystems engineering · 2026Article
- Development and optimization of a glycerol-based fed-batch strategy for the production of peroxidases with Komagataella phaffii under the PMicrobial cell factories · 2026Article
- Transcriptional regulation: efficient genetic engineering tools for non-conventional yeasts.FEMS yeast research · 2026Review
- Next-generation stress-inducible Komagataella phaffii promoter variants.Microbial cell factories · 2025Article
- Signaling pathways contributing to the transcriptional response to alkalinization in Komagataella phaffii: role of the transcription factors Crz1 and Rim101.Scientific reports · 2025Article
- Balancing Doses of EL222 and Light Improves Optogenetic Induction of Protein Production in Komagataella phaffii.Biotechnology and bioengineering · 2025Article
- The MFα signal sequence in yeast-based protein secretion: challenges and innovations'.Applied microbiology and biotechnology · 2025Review
- Rational design and characterization of enhanced alcohol-inducible synthetic promoters inApplied and environmental microbiology · 2025Article
- Chances and drawbacks of derepressed recombinant enzyme production in continuous cultivations withFrontiers in bioengineering and biotechnology · 2025Article
- Yeast vaccine production platform for human and animal infectious diseases.Frontiers in immunology · 2025Review
- Hansenula polymorpha methanol metabolism genes enhance recombinant protein production in Komagataella phaffi.AMB Express · 2024Article
- Bi-directionalized promoter systems allow methanol-free production of hard-to-express peroxygenases with Komagataella Phaffii.Microbial cell factories · 2024Article
- Recent progress on heterologous protein production in methylotrophic yeast systems.World journal of microbiology & biotechnology · 2024Review
- Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection.Microbial cell factories · 2024Article
- Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii.Microbial cell factories · 2024Article
- Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions.Microbial cell factories · 2023Article
- A genome-informed higher rank classification of the biotechnologically important fungal subphylumStudies in mycology · 2023Article
- Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris.Microbial cell factories · 2023Article
- Enabling growth-decoupledFrontiers in bioengineering and biotechnology · 2023Article
- Applicability of the heterologous yeast promoters for recombinant protein production in Pichia pastoris.Applied microbiology and biotechnology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris, Pp), Hansenula polymorpha (Hp), Candida boidinii (Cb) and Pichia methanolica (Pm) are widely used protein production platforms. Typically, strong, tightly regulated promoters of genes coding for their methanol utilization (MUT) pathways are used to drive heterologous gene expression. Despite highly similar open reading frames in the MUT pathways of the four yeasts, the regulation of the respective promoters varies strongly between species. While most endogenous Pp MUT promoters remain tightly repressed after depletion of a repressing carbon, Hp, Cb and Pm MUT promoters are derepressed to up to 70% of methanol induced levels, enabling methanol free production processes in their respective host background. Here, we have tested a series of orthologous promoters from Hp, Cb and Pm in Pp. Unexpectedly, when induced with methanol, the promoter of the HpMOX gene reached very similar expression levels as the strong methanol, inducible, and most frequently used promoter of the Pp alcohol oxidase 1 gene (P
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.