ReviewMetabolic engineering communications2020
Metabolic flux analysis of secondary metabolism in plants.
Review in Metabolic engineering communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Mainstreaming orphan millets for advancing climate smart agriculture to secure nutrition and health.Frontiers in plant science · 2022Pooled it
- Medicinal-plant immunity and specialized metabolism in plant-pathogen interactions: mechanisms and applications.Planta · 2026Review
- Organ-on-a-Chip and Microfluidic Plant Cell Culture Systems: The Next Frontier for Controlled Secondary Metabolite Production and Real-Time Metabolomic Monitoring.Plants (Basel, Switzerland) · 2026Review
- mFLIP: metabolic flux interval prediction.BMC bioinformatics · 2026Article
- Invasive Asian water moss (Frontiers in plant science · 2026Article
- Metabolic engineering of tobacco for heterologous production of rare ginsenosides CK and Rh2.Frontiers in plant science · 2026Article
- A voyage of reprogrammable metabolic bioengineering reshapes plant defense: from editing tools to synthetic systems.Frontiers in plant science · 2026Review
- Productive chaos and precision engineering: decoupling discovery from manufacturing to revolutionize plant-inspired therapeutics.Frontiers in plant science · 2026Article
- The biosynthesis and diversity of taxanes: From pathway elucidation to engineering and synthetic biology.Plant communications · 2025Review
- Unlocking theMolecules (Basel, Switzerland) · 2025Article
- A Guide to Metabolic Network Modeling for Plant Biology.Plants (Basel, Switzerland) · 2025Review
- RBI: a novel algorithm for regulatory-metabolic network model in designing the optimal mutant strain.PeerJ. Computer science · 2025Article
- Altitude-Dependent Morphophysiological, Anatomical, and Metabolomic Adaptations inPlants (Basel, Switzerland) · 2024Article
- Energy Metabolism Enhance Perylenequinone Biosynthesis inInternational journal of molecular sciences · 2024Article
- Microbial allies: exploring fungal endophytes for biosynthesis of terpenoid indole alkaloids.Archives of microbiology · 2024Review
- Connecting metabolome and phenotype: recent advances in functional metabolomics tools for the identification of bioactive natural products.Natural product reports · 2024Review
- Anatomical and Metabolome Features ofInternational journal of molecular sciences · 2024Article
- Transcriptome Analysis Reveals Candidate Genes Involved in Gibberellin-Induced Fruit Development inPlants (Basel, Switzerland) · 2023Article
- Current Status and De Novo Synthesis of Anti-Tumor Alkaloids inMetabolites · 2023Review
- Expression inheritance and constraints on cis- and trans-regulatory mutations underlying lotus color variation.Plant physiology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Numerous secondary metabolites from plants are important for their medicinal, nutraceutical or sensory properties. Recently, significant progress has been made in the identification of the genes and enzymes of plant secondary metabolic pathways. Hence, there is interest in using synthetic biology to enhance the production of targeted valuable metabolites in plants. In this article, we examine the contribution that metabolic flux analysis will have on informing the rational selection of metabolic engineering targets as well as analysis of carbon and energy efficiency. Compared to microbes, plants have more complex tissue, cellular and subcellular organization, making precise metabolite concentration measurements more challenging. We review different techniques involved in quantifying flux and provide examples illustrating the application of the techniques. For linear and branched pathways that lead to end products with low turnover, flux quantification is straightforward and doesn't require isotopic labeling. However, for metabolites synthesized via parallel pathways, there is a requirement for isotopic labeling experiments. If the fed isotopically labeled carbons don't scramble, one needs to apply transient label balancing methods. In the transient case, it is also necessary to measure metabolite concentrations. While flux analysis is not able to directly identify mechanisms of regulation, it is a powerful tool to examine flux distribution at key metabolic nodes in intermediary metabolism, detect flux to wasteful side pathways, and show how parallel pathways handle flux in wild-type and engineered plants under a variety of physiological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.