ArticleScientific reports2020
Dual-molecular barcode sequencing detects rare variants in tumor and cell free DNA in plasma.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Development and Performance Validation of a Comprehensive Liquid Biopsy Genotyping Panel for Pan-cancer Analysis.Annals of laboratory medicine · 2026Article
- Longitudinal genomic profiling of chemotherapy-related CHIP variants in patients with ovarian cancer.Frontiers in oncology · 2025Article
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- Duodenal Fluid Analysis as a Rewarding Approach to Detect Low-Abundance Mutations in Biliopancreatic Cancers.International journal of molecular sciences · 2024Article
- Comparison of diagnostic performance between Oncomine Dx target test and AmoyDx panel for detecting actionable mutations in lung cancer.Scientific reports · 2024Article
- Quantification of rare somatic single nucleotide variants by droplet digital PCR using SuperSelective primers.Scientific reports · 2023Article
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- Comparison between Three Different Techniques for the Detection of EGFR Mutations in Liquid Biopsies of Patients with Advanced Stage Lung Adenocarcinoma.International journal of molecular sciences · 2023Article
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- Correlation between circulating tumor DNA and carcinoembryonic antigen levels in patients with metastatic colorectal cancer.Cancer medicine · 2021Article
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- Evaluation of the Idylla ctEGFR mutation assay to detect EGFR mutations in plasma from patients with non-small cell lung cancers.Scientific reports · 2021Article
- Ultrasensitive detection of tumor-specific mutations in saliva of patients with oral cavity squamous cell carcinoma.Cancer · 2021Article
- Digital next-generation sequencing of cell-free DNA for pancreatic cancer.JGH open : an open access journal of gastroenterology and hepatology · 2021Article
- Next Generation Sequencing of Tumor and Matched Plasma Samples: Identification of Somatic Variants in ctDNA From Ovarian Cancer Patients.Frontiers in oncology · 2021Article
- Association of Mutation Profiles with Postoperative Survival in Patients with Non-Small Cell Lung Cancer.Cancers · 2020Article
- Primary Driver Mutations inCancers · 2020Article
- Droplet digital PCR-based detection of circulating tumor DNA from pediatric high grade and diffuse midline glioma patients.Neuro-oncology advancesArticle
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conventional next generation sequencing analysis has provided important insights into cancer genetics. However, the detection of rare (low allele fraction) variants remains difficult because of the error-prone nucleotide changes derived from sequencing/PCR errors. To eliminate the false-positive variants and detect genuine rare variants, sequencing technology combined with molecular barcodes will be useful. Here, we used the newly developed dual-molecular barcode technology (Ion AmpliSeq HD) to analyze somatic mutations in 24 samples (12 tumor tissues and 12 plasma) from 12 patients with biliary-pancreatic and non-small cell lung cancers. We compared the results between next generation sequencing analysis with or without molecular barcode technologies. The variant allele fraction (VAF) between non-molecular barcode and molecular barcode sequencing was correlated in plasma DNA (R
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