ArticleJournal of assisted reproduction and genetics2020
Improving the maturation rate of human oocytes collected ex vivo during the cryopreservation of ovarian tissue.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 2 syntheses or guidelines pooled it, 58 citations in OpenAlex.
- Fresh and cryopreserved ovarian tissue transplantation for preserving reproductive and endocrine function: a systematic review and individual patient data meta-analysis.Human reproduction update · 2022Pooled it
- Twenty-first century oocyte cryopreservation-in vitro maturation of immature oocytes from ovarian tissue cryopreservation in cancer patients: A systematic review.Women's health (London, England)Pooled it
- The ovulation trigger method affects gonadotropin concentrations and gonadotropin receptor expression during final oocyte maturation in women.Frontiers in endocrinology · 2026Article
- [Ovarian tissue cryopreservation and transplantation: a review of clinical progress in fertility preservation].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2025Review
- Transcriptional Changes in Cumulus Cells During In Vitro Maturation of Human Oocytes.Molecular reproduction and development · 2025Article
- Regulating human oocyte maturation in vitro: a hypothesis based on oocytes retrieved from small antral follicles during ovarian tissue cryopreservation.Journal of assisted reproduction and genetics · 2025Article
- Fertility preservation methods in children with the risk of female hypogonadism - possibilities and limitations.Pediatric endocrinology, diabetes, and metabolism · 2025Review
- New Insights on In Vitro Maturation of Oocytes for Fertility Preservation.International journal of molecular sciences · 2024Review
- [Reproductive Strategies for Declining Fertility: Fertility Preservation].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2024Review
- Successful pregnancy using immature oocytes retrieved from resected borderline ovarian tumor: a case report and literature review.Contraception and reproductive medicine · 2024Article
- In Vitro Maturation, In Vitro Oogenesis, and Ovarian Longevity.Reproductive sciences (Thousand Oaks, Calif.) · 2024Review
- Histological analysis of (antral) follicle density in ovarian cortex tissue attached to stripped endometriomas.Journal of assisted reproduction and genetics · 2024Article
- Moving the context of preserving fertility into an expanded domain.Journal of assisted reproduction and genetics · 2023Article
- In vitro maturation (IVM) of human immature oocytes: is it still relevant?Reproductive biology and endocrinology : RB&E · 2023Review
- Summary of the ISFP congress, Brussels, 10-12 November, 2022.Journal of assisted reproduction and genetics · 2023Review
- Oocyte diameter predicts the maturation rate of human immature oocytes collected ex vivo.Journal of assisted reproduction and genetics · 2022Article
- Closing the knowledge void between healthy gametes and healthy offspring.Journal of assisted reproduction and genetics · 2022Article
- Concentrations of oocyte secreted GDF9 and BMP15 decrease with MII transition during human IVM.Reproductive biology and endocrinology : RB&E · 2022Article
- The Improvement and Clinical Application of Human Oocyte In Vitro Maturation (IVM).Reproductive sciences (Thousand Oaks, Calif.) · 2022Review
- Shuttle Transfer of mRNA TranscriptsFrontiers in veterinary science · 2022Article
Corrections and comments
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Authors and funding
16 authors at 5 institutions in 5 countries.
Funding
Abstract
purposeThe aim of the present study was to improve the in vitro maturation (IVM) procedure using oocytes from surplus ovarian tissue after fertility preservation.
methodsTwenty-five patients aged 17-37 years were included in the study. Maturation was compared between oocytes collected in HEPES-buffered medium or saline, and we determined whether transport on ice prior to oocyte collection affected maturation. Two different IVM media were used that were supplemented with and without recombinant human midkine. Mature oocytes were assessed for aneuploidy using next-generation sequencing (NGS).
resultsOn average, 36 immature oocytes were collected from each patient (range 7-90, N = 895). Oocytes recovered from HEPES-buffered medium matured at a higher rate than oocytes recovered from saline (36% vs 26%, p < 0.01). Ovarian transportation on ice prior to the procedure negatively affected maturation compared with non-transported samples (42% vs 27%, p < 0.01). The addition of midkine improved maturation rate (34% vs 27%, p < 0.05). On average, 11 MII oocytes were obtained per patient (range 1-30). NGS of 53 MII oocytes and their first polar bodies indicated that 64% were euploid.
conclusionsThe study demonstrated unexpectedly high number of immature oocytes collected from surplus ovarian tissue without any stimulation. The overall MII rate was one in three, resulting in a total number of MII oocytes that was similar to the number obtained after ovarian stimulation. If these MII oocytes prove suitable for IVF, they will provide a substantial improvement in fertility preservation for patients and advance IVM as an interesting platform for further improvements in assisted reproduction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.