Evidence map›Paper›PMID 32095920›Full record

ArticleLasers in medical science2020

Photobiomodulation therapy improves human dental pulp stem cell viability and migration in vitro associated to upregulation of histone acetylation.

Ivana M Zaccara, Letícia B Mestieri, Emily F S Pilar, Maria S Moreira, Fabiana S Grecca, Manoela D Martins, Patrícia Maria Poli Kopper

Abstract read
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In one paragraph

Article in Lasers in medical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 1 pooled it
2.3field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.

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  8. Evaluation of photobiomodulation therapy (117 and 90s) on pain, regeneration, and epigenetic factors (HDAC 2, DNMT3a) expression following spinal cord injury in a rat model.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ivana M ZaccaraDentistry Graduate Program, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul - UFRGS, Rua Ramiro Barcelos, 2492, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
Letícia B MestieriDentistry Graduate Program, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul - UFRGS, Rua Ramiro Barcelos, 2492, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
Emily F S PilarDepartment of Experimental Pathology, Clinics Hospital of Porto Alegre, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, Rio Grande do Sul, Brazil.
Maria S MoreiraDepartment of Dentistry, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.
Fabiana S GreccaDentistry Graduate Program, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul - UFRGS, Rua Ramiro Barcelos, 2492, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
Manoela D MartinsDentistry Graduate Program, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul - UFRGS, Rua Ramiro Barcelos, 2492, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
Patrícia Maria Poli KopperDentistry Graduate Program, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul - UFRGS, Rua Ramiro Barcelos, 2492, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil. patricia.kopper@ufrgs.br.ORCID http://orcid.org/0000-0002-2514-6036
Universidade Federal do Rio Grande do Sul · BRUniversidade de São Paulo · BR

Funding

CSRD VA 1
6 · The paper itself

Abstract

This in vitro study evaluated the role of photobiomodulation therapy (PBMT) on viability and migration of human dental pulp stem cells (hDPSCs) and its association to epigenetic mechanisms such as histone acetylation. The hDPSCs were characterized and assigned into control and PBMT groups. For the PBMT, five laser irradiations at 6-h intervals were performed using a continuous-wave InGaAlP diode laser. Viability (MTT), migration (scratch), and histone acetylation H3 (H3K9ac immunofluorescence) were evaluated immediately after the last irradiation. PBMT significantly increased the viability (P = 0.004). Also, PBMT group showed significantly increased migration of cells in the wound compared to the control in 6 h (P = 0.002), 12 h (P = 0.014) and 18 h (P = 0.083) being faster than the control, which only finished the process at 24 h. PBMT induced epigenetic modifications in hDPSC due to increased histone acetylation (P = 0.001). PBMT increased viability and migration of hDPSCs, which are related with the upregulation of histone acetylation and could be considered a promising adjuvant therapy for regenerative endodontic treatment.

Indexed as

Low-Level Light TherapyAcetylationCell MovementCell SurvivalDental PulpHistonesHumansStem CellsUp-RegulationHistonesDental pulpEpigenetic regulationLaser phototherapyPhotobiomodulation therapyStem cells

Identifiers

PMID32095920
OpenAlexW3007303729

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.