ArticleScience advances2020
Osteogenic preconditioning in perfusion bioreactors improves vascularization and bone formation by human bone marrow aspirates.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
29 citing papers in PubMed, 44 citations in OpenAlex.
- Biophysical signal-driven scaffold design for stem cell-guided osteochondral regeneration.Bioactive materials · 2026Review
- Gelatin-Polyvinyl Alcohol Microspheres for Controlled and Sustained Release of BMP-2 and VEGF Enhance Osteogenic and Angiogenic Cell Differentiation.Gels (Basel, Switzerland) · 2026Article
- Divide-and-conquer strategy with engineered ossification center organoids for rapid bone healing through developmental cell recruitment.Nature communications · 2025Article
- Design and construction of a low-cost compressive loading and perfusion flow bioreactor.HardwareX · 2024Article
- Functionalized Annealed Microgels for Spatial Control of Osteogenic and Chondrogenic Differentiation.Advanced functional materials · 2024Article
- Investigation of osteogenesis and angiogenesis in perfusion bioreactors using improved multi-layer PCL-nHA-nZnO electrospun scaffolds.Biotechnology letters · 2023Article
- 3D Bioprinting in Otolaryngology: A Review.Advanced healthcare materials · 2023Review
- Tuning the Microenvironment to Create Functionally Distinct Mesenchymal Stromal Cell Spheroids.Annals of biomedical engineering · 2023Article
- Unique osteogenic profile of bone marrow stem cells stimulated in perfusion bioreactor is Rho-ROCK-mediated contractility dependent.Bioengineering & translational medicine · 2023Article
- Remote control of the recruitment and capture of endogenous stem cells by ultrasound for in situ repair of bone defects.Bioactive materials · 2023Article
- Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering.Computational and structural biotechnology journal · 2023Review
- Macromolecular crowding and decellularization method increase the growth factor binding potential of cell-secreted extracellular matrices.Frontiers in bioengineering and biotechnology · 2023Article
- Mesenchymal stromal cell spheroids in sulfated alginate enhance muscle regeneration.Acta biomaterialia · 2023Article
- Msx1Nature communications · 2022Article
- Characterization of Induction and Targeting of Senescent Mesenchymal Stromal Cells.Tissue engineering. Part C, Methods · 2022Article
- An instantly fixable and self-adaptive scaffold for skull regeneration by autologous stem cell recruitment and angiogenesis.Nature communications · 2022Article
- Engineered Cell-Secreted Extracellular Matrix Modulates Cell Spheroid Mechanosensing and Amplifies Their Response to Inductive Cues for the Formation of Mineralized Tissues.Advanced healthcare materials · 2022Article
- Advanced PLGA hybrid scaffold with a bioactive PDRN/BMP2 nanocomplex for angiogenesis and bone regeneration using human fetal MSCs.Science advances · 2021Article
- Sulfated Alginate Hydrogels Prolong the Therapeutic Potential of MSC Spheroids by Sequestering the Secretome.Advanced healthcare materials · 2021Article
- Osteogenic and Angiogenic Synergy of Human Adipose Stem Cells and Human Umbilical Vein Endothelial Cells Cocultured in a Modified Perfusion Bioreactor.Organogenesis · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Cell-derived extracellular matrix (ECM) provides a niche to promote osteogenic differentiation, cell adhesion, survival, and trophic factor secretion. To determine whether osteogenic preconditioning would improve the bone-forming potential of unfractionated bone marrow aspirate (BMA), we perfused cells on ECM-coated scaffolds to generate naïve and preconditioned constructs, respectively. The composition of cells selected from BMA was distinct on each scaffold. Naïve constructs exhibited robust proangiogenic potential in vitro, while preconditioned scaffolds contained more mesenchymal stem/stromal cells (MSCs) and endothelial cells (ECs) and exhibited an osteogenic phenotype. Upon implantation into an orthotopic calvarial defect, BMA-derived ECs were present in vessels in preconditioned implants, resulting in robust perfusion and greater vessel density over the first 14 days compared to naïve implants. After 10 weeks, human ECs and differentiated MSCs were detected in de novo tissues derived from naïve and preconditioned scaffolds. These results demonstrate that bioreactor-based preconditioning augments the bone-forming potential of BMA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.