ArticlePeerJ2020
Comprehensive transcriptome analysis of reference genes for fruit development of
Article in PeerJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (Current issues in molecular biology · 2025Article
- A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression.Forestry research · 2024Article
- Selection and validation of appropriate reference genes for RT-qPCR analysis of Nitraria sibirica under various abiotic stresses.BMC plant biology · 2022Article
- Determination of reliable reference genes for gene expression studies in Chinese chive (Allium tuberosum) based on the transcriptome profiling.Scientific reports · 2021Article
- Genome-Wide Identification and Co-Expression Analysis ofFrontiers in genetics · 2021Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
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Abstract
backgroundQuantitativereal-time reverse transcriptase polymerase chain reaction is the common method to quantify relative gene expression. Normalizating using reliable genes is critical in correctly interpreting expression data from qRT-PCR.
methodsWe selected eight candidate reference genes based on RNA-seq database analysis, and ranked expression stability using statistical algorithms GeNorm, NormFinder, BestKeeper and ReFinder. Finally, The nine genes related to the anthocyanin synthesis pathway of Euscaphis konishii were used to verify the suitability of reference gene.
resultsThe results showed that the stability of EkUBC23, EkCYP38 and EkGAPDH2 was better, and the low expression reference genes (EkUBC23 and EkCYP38) were favourable for quantifying low expression target genes, while the high expression reference gene (EkGAPDH2) was beneficial for quantifying high expression genes. In this study, we present the suitable reference genes for fruit development of Euscaphis konishii based on transcriptome data, our study will contribute to further studies in molecular biology and gene function on
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