Evidence map›Paper›PMID 32094922›Full record

ArticleNature chemical biology2020

Endosome motility defects revealed at super-resolution in live cells using HIDE probes.

Aarushi Gupta, Felix Rivera-Molina, Zhiqun Xi, Derek Toomre, Alanna Schepartz

Open access · greenAbstract read
In one paragraph

Article in Nature chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Bioorthogonal Chemistry in Cellular Organelles.Topics in current chemistry (Cham) · 2023
    Review
  6. Article
  7. Lipid Expansion Microscopy.Journal of the American Chemical Society · 2022
    Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Article
  13. Quantitative Imaging of BiochemistryACS central science · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Aarushi GuptaDepartment of Chemistry, Yale University, New Haven, CT, USA.
Felix Rivera-MolinaDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.
Zhiqun XiDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.
Derek ToomreDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT, USA. derek.toomre@yale.edu.ORCID http://orcid.org/0000-0002-6827-8127
Alanna SchepartzDepartment of Chemistry, Yale University, New Haven, CT, USA. schepartz@berkeley.edu.ORCID http://orcid.org/0000-0003-2127-3932
Yale University · US

Funding

Yale Clinical and Translational Science Award (U Component)UL1TR001863 · NCATS · YALE UNIVERSITY · PI John H. Krystal, LUCILA OHNO-MACHADO · 2016 to 2026
$102.9M
Yale Diabetes Research CenterP30DK045735 · NIDDK · YALE UNIVERSITY · PI Richard A Flavell · 1993 to 2026
$44.0M
Fluorescence tools that illuminate biology and inspire translationR35GM134963 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Alanna Schepartz · 2020 to 2026
$4.7M
Enabling Nanoscale Dynamic Imaging of Vesicles and OrganellesR01GM118486 · NIGMS · YALE UNIVERSITY · PI BADDELEY, DAVID, BEWERSDORF, JOERG · 2016 to 2019
$3.4M
A super-resolution microscope for research and educationS10OD020142 · OD · YALE UNIVERSITY · PI NATHANSON, MICHAEL H · 2015 to 2015
$600k
Expanding the HIDE nanoscopy toolbox: More organelles, colors, and modalitiesR01GM131372 · NIGMS · YALE UNIVERSITY · PI SCHEPARTZ, ALANNA · 2019 to 2019
$299k
NCATS NIH HHS UL1 TR001863NIDDK NIH HHS P30 DK045735NIGMS NIH HHS R01 GM118486NIGMS NIH HHS R01 GM131372NIGMS NIH HHS R35 GM134963NIH HHS S10 OD020142ODCDC CDC HHS S10 OD020142U.S. Department of Health & Human Services | National Institutes of Health (NIH) 5T32GM06754U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01GM118486U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R01GM131372Wellcome TrustWellcome Trust (Wellcome) 095927/A/11/Z
6 · The paper itself

Abstract

We report new lipid-based, high-density, environmentally sensitive (HIDE) probes that accurately and selectively image endo-lysosomes and their dynamics at super-resolution for extended times. Treatment of live cells with the small molecules DiIC

Indexed as

Biological TransportCarbocyaninesCarrier ProteinsCholesterolEndosomesFibroblastsFluorescent DyesHeLa CellsHumansLysosomesMembrane GlycoproteinsMicroscopy, FluorescenceProtein Transport3,3'-dihexadecylindocarbocyanineCarbocyaninesCarrier ProteinsCholesterolFluorescent DyesMembrane Glycoproteins

Identifiers

PMID32094922
PMCPMC7176048
OpenAlexW3007368663

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.